• Immunofluorescence analysis of paraffin-embedded mouse brain using Alpha Internexin Rabbit monoclonal antibody (STJ11101907) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat brain using Alpha Internexin Rabbit monoclonal antibody (STJ11101907) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Alpha Internexin in paraffin-embedded mouse brain tissue using Alpha Internexin Rabbit monoclonal antibody (STJ11101907) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Alpha Internexin in paraffin-embedded human appendix tissue using Alpha Internexin Rabbit monoclonal antibody (STJ11101907) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Alpha Internexin in paraffin-embedded human brain tissue using Alpha Internexin Rabbit monoclonal antibody (STJ11101907) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Alpha Internexin in paraffin-embedded rat brain tissue using Alpha Internexin Rabbit monoclonal antibody (STJ11101907) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Alpha Internexin in paraffin-embedded human colon tissue using Alpha Internexin Rabbit monoclonal antibody (STJ11101907) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using Alpha Internexin Rabbit monoclonal antibody (STJ11101907) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-INA antibody (350-450) [S7MR] (STJ11101907)

SKU:
STJ11101907

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: INA
Gene ID: 9118
Uniprot ID: AINX_HUMAN
Immunogen Region: 350-450
Specificity: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human Alpha Internexin (Q16352).
Immunogen Sequence: EVAGYQDSIGQLENDLRNTK SEMARHLREYQDLLNVKMAL DIEIAAYRKLLEGEETRFST SGLSISGLNPLPNPSYLLPP RILSATTSKVSSTGLSLKKE E
Tissue Specificity Found predominantly in adult CNS.
Post Translational Modifications O-glycosylated.
Function Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NEFL to form the filamentous backbone to which NEFM and NEFH attach to form the cross-bridges. May also cooperate with the neuronal intermediate filament protein PRPH to form filamentous networks.
Protein Name Alpha-Internexin
Alpha-Inx
66 Kda Neurofilament Protein
Nf-66
Neurofilament-66
Neurofilament 5
Alternative Antibody Names Anti-Alpha-Internexin antibody
Anti-Alpha-Inx antibody
Anti-66 Kda Neurofilament Protein antibody
Anti-Nf-66 antibody
Anti-Neurofilament-66 antibody
Anti-Neurofilament 5 antibody
Anti-INA antibody
Anti-NEF5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance