Anti-IL4I1 antibody (22-567) (STJ110676)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal IL4I1 (22-567) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | IL4I1 |
| Gene ID | 259307 |
| Uniprot ID | OXLA_HUMAN |
| Immunogen Region | 22-567 |
| Immunogen Sequence | QDWKAERSQDPFEKCMQDPD YEQLLKVVTWGLNRTLKPQR VIVVGAGVAGLVAAKVLSDA GHKVTILEADNRIGGRIFTY RDQNTGWIGELGAMRMPSSH RILHKLCQGLGLNLTKFTQY DKNTWTEVHEVKLRNYVVEK VPEKLGYALRPQEKGHSPED IYQMALNQALKDLKALGCRK AMKKFERHTLLEYLLGEGN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 22-220 of human IL4I1 (NP_690863.1). |
Additional Info
| Tissue Specificity | Primarily found in immune tissues, with the highest expression in lymph nodes and spleen. Present in germinal center macrophages and inflammatory myeloid cells and antigen-presenting cells (at protein level). Also present in spermatozoa (at protein level). Highly expressed in primary mediastinal large B-cell lymphoma, a specific subtype of diffuse large B-cell lymphoma. Expressed by neoplastic cells of several B-cell lymphomas and by tumor-associated macrophages. |
| Post Translational Modifications | N-glycosylated. |
| Function | Secreted L-amino-acid oxidase that acts as a key immunoregulator. Has preference for L-aromatic amino acids: converts phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp) to phenylpyruvic acid (PP), hydroxyphenylpyruvic acid (HPP), and indole-3-pyruvic acid (I3P), respectively. Also has weak L-arginine oxidase activity. Acts as a negative regulator of anti-tumor immunity by mediating Trp degradation via an indole pyruvate pathway that activates the transcription factor AHR. IL4I1-mediated Trp catabolism generates I3P, giving rise to indole metabolites (indole-3-acetic acid (IAA) and indole-3-aldehyde (I3A)) and kynurenic acid, which act as ligands for AHR, a ligand-activated transcription factor that plays important roles in immunity and cancer. AHR activation by indoles following IL4I1-mediated Trp degradation enhances tumor progression by promoting cancer cell motility and suppressing adaptive immunity. Also has an immunoregulatory function in some immune cells, probably by mediating Trp degradation and promoting downstream AHR activation: inhibits T-cell activation and proliferation, promotes the differentiation of naive CD4(+) T-cells into FOXP3(+) regulatory T-cells (Treg) and regulates the development and function of B-cells. Also regulates M2 macrophage polarization by inhibiting T-cell activation. Also has antibacterial properties by inhibiting growth of Gram negative and Gram positive bacteria through the production of NH4(+) and H2O2. |
| Protein Name | L-Amino-Acid OxidaseLaaoLaoInterleukin-4-Induced Protein 1Il4-Induced Protein 1Hil4i1Protein Fig-1Hfig1 |
| Database Links | Reactome: R-HSA-8964208 |
| Cellular Localisation | SecretedLysosomeCytoplasmic VesicleSecretory VesicleAcrosomeSecreted At The Immunological Synapse |
| Alternative Antibody Names | Anti-L-Amino-Acid Oxidase antibodyAnti-Laao antibodyAnti-Lao antibodyAnti-Interleukin-4-Induced Protein 1 antibodyAnti-Il4-Induced Protein 1 antibodyAnti-Hil4i1 antibodyAnti-Protein Fig-1 antibodyAnti-Hfig1 antibodyAnti-IL4I1 antibodyAnti-FIG1 antibodyAnti-UNQ636 antibodyAnti-PRO1265 antibody |
Information sourced from Uniprot.org