• Flow cytometry was performed with Jurkat cells.
  • Western blot analysis of recombinant Human IL2 Protein using IL2 Rabbit monoclonal antibody (STJ11103947) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 250pg- 1ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from Jurkat cells, using IL2 Rabbit monoclonal antibody (STJ11103947) at 1:1000 dilution. Jurkat cells were treated by TPA (40 nM) , A23187 (2 Mu M) and Brefeldin A (300 ng/ ml) for 0-24hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-IL2 antibody (1-153) [S3947RM] (STJ11103947)

SKU:
STJ11103947

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Host: Rabbit
Applications: WB/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Interleukin-2 (1-153) is suitable for use in Western Blot and Flow Cytometry research applications.
Clonality: Monoclonal
Clone ID: S3947RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
FC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL2
Gene ID: 3558
Uniprot ID: IL2_HUMAN
Immunogen Region: 1-153
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human IL2 (NP_000577.2).
Immunogen Sequence: MYRMQLLSCIALSLALVTNS APTSSSTKKTQLQLEHLLLD LQMILNGINNYKNPKLTRML TFKFYMPKKATELKHLQCLE EELKPLEEVLNLAQSKNFHL RPRDLISNINVIVLELKGSE TTFMCEYADETATIVEFLNR WITFCQSIISTLT
Function Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells.
Protein Name Interleukin-2
Il-2
T-Cell Growth Factor
Tcgf
Aldesleukin
Database Links Reactome: R-HSA-5673001
Reactome: R-HSA-8877330
Reactome: R-HSA-9020558
Reactome: R-HSA-912526
Cellular Localisation Secreted
Alternative Antibody Names Anti-Interleukin-2 antibody
Anti-Il-2 antibody
Anti-T-Cell Growth Factor antibody
Anti-Tcgf antibody
Anti-Aldesleukin antibody
Anti-IL2 antibody

Information sourced from Uniprot.org

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