• Western blot analysis of extracts of THP-1 cells, using IL1 Beta antibody (STJ11101739) at 1:1000 dilution. THP-1 cells were treated by LPS (1 Mu g/mL) at 37 °C for 8 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunofluorescence analysis of THP-1 cells using IL1 Beta antibody (STJ11101739). THP-1 cells were treated by LPS (1 Mu g/mL) at 37 °C for 8 hours. Blue: DAPI for nuclear staining.

Anti-IL1B antibody (117-269) [S9MR] (STJ11101739)

SKU:
STJ11101739

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-IL1 beta (117-269) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL1B
Gene ID: 3553
Uniprot ID: IL1B_HUMAN
Immunogen Region: 117-269
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 117-269 of human IL1 Beta (NP_000567.1).
Immunogen Sequence: APVRSLNCTLRDSQQKSLVM SGPYELKALHLQGQDMEQQV VFSMSFVQGEESNDKIPVAL GLKEKNLYLSCVLKDDKPTL QLESVDPKNYPKKKMEKRFV FNKIEINNKLEFESAQFPNW YISTSQAENMPVFLGGTKGG QDITDFTMQFVSS
Tissue Specificity Expressed in activated monocytes/macrophages (at protein level).
Post Translational Modifications Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. (Microbial infection) Cleavage by S.pyogenes cysteine protease SpeB promotes its activation independently of CASP1.
Function Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection.
Protein Name Interleukin-1 Beta
Il-1 Beta
Catabolin
Database Links Reactome: R-HSA-448706
Reactome: R-HSA-5620971
Reactome: R-HSA-5660668
Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-9020702
Reactome: R-HSA-9660826
Cellular Localisation Cytoplasm
Cytosol
Secreted
Lysosome
Extracellular Exosome
The Precursor Is Cytosolic
In Response To Inflammasome-Activating Signals
Such As Atp For Nlrp3 Inflammasome Or Bacterial Flagellin For Nlrc4 Inflammasome
Cleaved And Secreted
Mature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) Pore
In Contrast
The Precursor Form Is Not Released
Due To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During Maturation
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
Alternative Antibody Names Anti-Interleukin-1 Beta antibody
Anti-Il-1 Beta antibody
Anti-Catabolin antibody
Anti-IL1B antibody
Anti-IL1F2 antibody

Information sourced from Uniprot.org

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