| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-IL12B/IL12p40 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000. WB: Approx 37kDa band observed in Human Liver, Skin and Tonsil lysates (calculated MW of 37.2kDa according to NP_002178.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | IL12B |
| Gene ID: | 3593 |
| Uniprot ID: | IL12B_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_002178.2 |
| Immunogen Sequence: | QGKSKREKKDRVFTD |
| Function | Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. |
| Protein Name | Interleukin-12 Subunit Beta Il-12b Cytotoxic Lymphocyte Maturation Factor 40 Kda Subunit Clmf P40 Il-12 Subunit P40 Nk Cell Stimulatory Factor Chain 2 Nksf2 |
| Database Links | Reactome: R-HSA-6783783 Reactome: R-HSA-6785807 Reactome: R-HSA-9020591 Reactome: R-HSA-9020933 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Interleukin-12 Subunit Beta antibody Anti-Il-12b antibody Anti-Cytotoxic Lymphocyte Maturation Factor 40 Kda Subunit antibody Anti-Clmf P40 antibody Anti-Il-12 Subunit P40 antibody Anti-Nk Cell Stimulatory Factor Chain 2 antibody Anti-Nksf2 antibody Anti-IL12B antibody Anti-NKSF2 antibody |
Information sourced from Uniprot.org