• Western blot analysis of recombinant Human IL12A/NKSF1 Protein, using IL12A Rabbit polyclonal antibody (STJ11103288) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of lysates from RAW264. 7 cells, using IL12A Rabbit polyclonal antibody (STJ11103288) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-IL12A antibody (50-150) (STJ11103288)

SKU:
STJ11103288

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL12A (50-150) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL12A
Gene ID: 3592
Uniprot ID: IL12A_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human IL12A (NP_000873.2).
Immunogen Sequence: LDHLSLARNLPVATPDPGMF PCLHHSQNLLRAVSNMLQKA RQTLEFYPCTSEEIDHEDIT KDKTSTVEACLPLELTKNES CLNSRETSFITNGSCLASRK T
Function Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity. Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits. Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2. In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes. As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and functions also as an immune-suppressive cytokine. Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers. Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites.
Protein Name Interleukin-12 Subunit Alpha
Il-12a
Cytotoxic Lymphocyte Maturation Factor 35 Kda Subunit
Clmf P35
Il-12 Subunit P35
Nk Cell Stimulatory Factor Chain 1
Nksf1
Database Links Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-8984722
Reactome: R-HSA-9020591
Cellular Localisation Secreted
Alternative Antibody Names Anti-Interleukin-12 Subunit Alpha antibody
Anti-Il-12a antibody
Anti-Cytotoxic Lymphocyte Maturation Factor 35 Kda Subunit antibody
Anti-Clmf P35 antibody
Anti-Il-12 Subunit P35 antibody
Anti-Nk Cell Stimulatory Factor Chain 1 antibody
Anti-Nksf1 antibody
Anti-IL12A antibody
Anti-NKSF1 antibody

Information sourced from Uniprot.org

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