| Host: |
Rabbit |
| Applications: |
WB/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: |
IL12A |
| Gene ID: |
3592 |
| Uniprot ID: |
IL12A_HUMAN |
| Immunogen Region: |
23-219 aa |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 23-219 of human IL12A (NP_001384921.1). |
| Immunogen Sequence: |
RNLPVATPDPGMFPCLHHSQ NLLRAVSNMLQKARQTLEFY PCTSEEIDHEDITKDKTSTV EACLPLELTKNESCLNSRET SFITNGSCLASRKTSFMMAL CLSSIYEDLKMYQVEFKTMN AKLLMDPKRQIFLDQNMLAV IDELMQALNFNSETVPQKSS LEEPDFYKTKIKLCILLHAF RIRAVTIDRVMSYLNAS |
| Function | Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity. Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits. Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2. In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes. As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and functions also as an immune-suppressive cytokine. Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers. Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites. |
| Protein Name | Interleukin-12 Subunit AlphaIl-12aCytotoxic Lymphocyte Maturation Factor 35 Kda SubunitClmf P35Il-12 Subunit P35Nk Cell Stimulatory Factor Chain 1Nksf1 |
| Database Links | Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-8984722Reactome: R-HSA-9020591 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Interleukin-12 Subunit Alpha antibodyAnti-Il-12a antibodyAnti-Cytotoxic Lymphocyte Maturation Factor 35 Kda Subunit antibodyAnti-Clmf P35 antibodyAnti-Il-12 Subunit P35 antibodyAnti-Nk Cell Stimulatory Factor Chain 1 antibodyAnti-Nksf1 antibodyAnti-IL12A antibodyAnti-NKSF1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
