| Host: | Mouse |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Interleukin-10 is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 3C12C12 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2a |
| Formulation: | Liquid in PBS containing 0.03% Sodium Azide. |
| Purification: | Affinity purification |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 ELISA 1:10000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | IL10 |
| Gene ID: | 3586 |
| Uniprot ID: | IL10_HUMAN |
| Specificity: | IL-10 Monoclonal Antibody detects endogenous levels of IL-10 protein. |
| Immunogen: | Purified recombinant fragment of human IL-10 expressed in E. Coli. |
| Function | Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling. |
| Protein Name | Interleukin-10 Il-10 Cytokine Synthesis Inhibitory Factor Csif |
| Database Links | Reactome: R-HSA-6783783 Reactome: R-HSA-6785807 Reactome: R-HSA-8950505 Reactome: R-HSA-9662834 Reactome: R-HSA-9664323 Reactome: R-HSA-9725370 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Interleukin-10 antibody Anti-Il-10 antibody Anti-Cytokine Synthesis Inhibitory Factor antibody Anti-Csif antibody Anti-IL10 antibody |
Information sourced from Uniprot.org