Anti-IL10 antibody (1-100) [S5MR] (STJ11102745)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal IL10 (1-100) antibody for use in WB, IF, ICC and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S5MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | IL10 |
| Gene ID | 3586 |
| Uniprot ID | IL10_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MHSSALLCCLVLLTGVRASP GQGTQSENSCTHFPGNLPNM LRDLRDAFSRVKTFFQMKDQ LDNLLLKESLLEDFKGYLGC QALSEMIQFYLEEVMPQAEN |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IL10 (P22301). |
Additional Info
| Tissue Specificity | Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. |
| Function | Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling. |
| Protein Name | Interleukin-10Il-10Cytokine Synthesis Inhibitory FactorCsif |
| Database Links | Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-8950505Reactome: R-HSA-9662834Reactome: R-HSA-9664323Reactome: R-HSA-9725370 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Interleukin-10 antibodyAnti-Il-10 antibodyAnti-Cytokine Synthesis Inhibitory Factor antibodyAnti-Csif antibodyAnti-IL10 antibody |
Information sourced from Uniprot.org