• Western blot analysis of recombinant Mouse IL-1 alpha Protein, using IL-1 alpha Rabbit monoclonal antibody (STJ11104952) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-IL-1 alpha antibody (115-270) [S4952RM] (STJ11104952)

SKU:
STJ11104952

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-IL-1 alpha (115-270) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4952RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Il1a
Gene ID: 16175
Uniprot ID: IL1A_MOUSE
Immunogen Region: 115-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 115-270 of mouse IL-1 alpha (NP_034684.2).
Immunogen Sequence: SAPYTYQSDLRYKLMKLVRQ KFVMNDSLNQTIYQDVDKHY LSTTWLNDLQQEVKFDMYAY SSGGDDSKYPVTLKISDSQL FVSAQGEDQPVLLKELPETP KLITGSETDLIFFWKSINSK NYFTSAAYPELFIATKEQSR VHLARGLPSMTDFQIS
Post Translational Modifications Acetylated within its nuclear localization sequence, which impacts subcellular localization. Proteolytic processed by CAPN1 in a calcium-dependent manner. Cleavage from 31 kDa precursor to 18 kDa biologically active molecules. Phosphorylated. Phosphorylation greatly enhances susceptibility to digestion and promotes the conversion of pre-IL1A alpha to the biologically active IL1A.
Function Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex. Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4. In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways. Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage. In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as signal for genotoxic stress without loss of cell integrity.
Protein Name Interleukin-1 Alpha
Il-1 Alpha
Database Links Reactome: R-MMU-448706
Reactome: -MMU-5620971
Reactome: -MMU-9020702
Cellular Localisation Nucleus
Cytoplasm
Secreted
The Lack Of A Specific Hydrophobic Segment In The Precursor Sequence Suggests That Il-1 Is Released By Damaged Cells Or Is Secreted By A Mechanism Differing From That Used For Other Secretory Proteins
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion
Recruited To Dna Damage Sites And Secreted After Genotoxic Stress
Alternative Antibody Names Anti-Interleukin-1 Alpha antibody
Anti-Il-1 Alpha antibody
Anti-Il1a antibody

Information sourced from Uniprot.org

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