• Western blot analysis of extracts of mouse kidney, using IGFBP5 antibody (STJ114325) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of mouse uterus, using IGFBP5 rabbit polyclonal antibody (STJ114325) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of Rat kidney, using IGFBP5 rabbit polyclonal antibody (STJ114325) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using IGFBP5 antibody (STJ114325) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using IGFBP5 antibody (STJ114325) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using IGFBP5 rabbit polyclonal antibody (STJ114325) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using IGFBP5 rabbit polyclonal antibody (STJ114325) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using IGFBP5 rabbit polyclonal antibody (STJ114325) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-IGFBP5 antibody (68-167) (STJ114325)

SKU:
STJ114325

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IGFBP5 (68-167) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IGFBP5
Gene ID: 3488
Uniprot ID: IBP5_HUMAN
Immunogen Region: 68-167
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 68-167 of human IGFBP5 (NP_000590.1).
Immunogen Sequence: GVYTERCAQGLRCLPRQDEE KPLHALLHGRGVCLNEKSYR EQVKIERDSREHEEPTTSEM AEETYSPKIFRPKHTRISEL KAEAVKKDRRKKLTQSKFVG
Tissue Specificity Osteosarcoma, and at lower levels in liver, kidney and brain.
Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Protein Name Insulin-Like Growth Factor-Binding Protein 5
Ibp-5
Igf-Binding Protein 5
Igfbp-5
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative Antibody Names Anti-Insulin-Like Growth Factor-Binding Protein 5 antibody
Anti-Ibp-5 antibody
Anti-Igf-Binding Protein 5 antibody
Anti-Igfbp-5 antibody
Anti-IGFBP5 antibody
Anti-IBP5 antibody

Information sourced from Uniprot.org

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