• Western blot analysis of extracts from wild type (WT) and IGFBP5 knockout (KO) 293T cells, using IGFBP5 antibody (STJ11104514) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using IGFBP5 antibody (STJ11104514) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-IGFBP5 antibody (21-272) (STJ11104514)

SKU:
STJ11104514

£129.50 - £642.50
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IGFBP5
Gene ID: 3488
Uniprot ID: IBP5_HUMAN
Immunogen Region: 21-272
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 21-272 of human IGFBP5 (NP_000590.1).
Immunogen Sequence: LGSFVHCEPCDEKALSMCPP SPLGCELVKEPGCGCCMTCA LAEGQSCGVYTERCAQGLRC LPRQDEEKPLHALLHGRGVC LNEKSYREQVKIERDSREHE EPTTSEMAEETYSPKIFRPK HTRISELKAEAVKKDRRKKL TQSKFVGGAENTAHPRIISA PEMRQESEQGPCRRHMEASL QELKASPRMVPRAVYLPNCD RKGFYKRKQCKPSRGRKRGI CWCVDKYGMKLPGMEYVDG
Tissue Specificity Osteosarcoma, and at lower levels in liver, kidney and brain.
Post Translational Modifications Cleaved by C1S in extracellular space.
Function Multifunctional protein that plays a critical role in regulating the availability of IGFs to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Increases the cell proliferation of osteoblasts, intestinal smooth muscle cells and neuroblastoma cells. Enhances adhesion and survival of epithelial cells but decreases adhesion of mesenchymal cells. Once secreted, acts as a major mediator of mTORC1-dependent feedback inhibition of IGF1 signaling. Plays also a role in the induction of extracellular matrix (ECM) production and deposition independently of its nuclear translocation and binding to IGFs. Acts itself as a growth factor that can act independently of IGFs to regulate bone formation. Acts as a ligand for the ROR1 receptor which triggers formation of ROR1/HER2 heterodimer to enhance CREB oncogenic signaling.
Protein Name Insulin-Like Growth Factor-Binding Protein 5
Ibp-5
Igf-Binding Protein 5
Igfbp-5
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Cytoplasm
Nucleus
Alternative Antibody Names Anti-Insulin-Like Growth Factor-Binding Protein 5 antibody
Anti-Ibp-5 antibody
Anti-Igf-Binding Protein 5 antibody
Anti-Igfbp-5 antibody
Anti-IGFBP5 antibody
Anti-IBP5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance