• Western blot analysis of extracts from wild type (WT) and IGFBP5 knockout (KO) 293T cells, using IGFBP5 antibody (STJ11104514) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using IGFBP5 antibody (STJ11104514) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-IGFBP5 antibody (21-272) (STJ11104514)

SKU:
STJ11104514

£129.50 - £639.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Insulin-Like Growth Factor-Binding Protein 5 (21-272) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IGFBP5
Gene ID: 3488
Uniprot ID: IBP5_HUMAN
Immunogen Region: 21-272
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-272 of human IGFBP5 (NP_000590.1).
Immunogen Sequence: LGSFVHCEPCDEKALSMCPP SPLGCELVKEPGCGCCMTCA LAEGQSCGVYTERCAQGLRC LPRQDEEKPLHALLHGRGVC LNEKSYREQVKIERDSREHE EPTTSEMAEETYSPKIFRPK HTRISELKAEAVKKDRRKKL TQSKFVGGAENTAHPRIISA PEMRQESEQGPCRRHMEASL QELKASPRMVPRAVYLPNCD RKGFYKRKQCKPSRGRKRGI CWCVDKYGMKLPGMEYVDG
Tissue Specificity Osteosarcoma, and at lower levels in liver, kidney and brain.
Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Protein Name Insulin-Like Growth Factor-Binding Protein 5
Ibp-5
Igf-Binding Protein 5
Igfbp-5
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative Antibody Names Anti-Insulin-Like Growth Factor-Binding Protein 5 antibody
Anti-Ibp-5 antibody
Anti-Igf-Binding Protein 5 antibody
Anti-Igfbp-5 antibody
Anti-IGFBP5 antibody
Anti-IBP5 antibody

Information sourced from Uniprot.org

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