• Western blot analysis of extracts of various cell lines, using IGFBP3 antibody (STJ118493) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using IGFBP3 antibody (STJ118493) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-IGFBP3 antibody (100-200) (STJ118493)

SKU:
STJ118493

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IGFBP3 (100-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IGFBP3
Gene ID: 3486
Uniprot ID: IBP3_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human IGFBP3 (NP_000589.2).
Immunogen Sequence: EARPLQALLDGRGLCVNASA VSRLRAYLLPAPPAPGNASE SEEDRSAGSVESPSVSSTHR VSDPKFHPLHSKIIIIKKGH AKDSQRYKVDYESQSTDTQN F
Tissue Specificity Expressed by most tissues. Present in plasma.
Post Translational Modifications Phosphorylated by FAM20C in the extracellular medium.
Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Inhibits the positive effect of humanin on insulin sensitivity. Promotes testicular germ cell apoptosis.
Protein Name Insulin-Like Growth Factor-Binding Protein 3
Ibp-3
Igf-Binding Protein 3
Igfbp-3
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-6803211
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative Antibody Names Anti-Insulin-Like Growth Factor-Binding Protein 3 antibody
Anti-Ibp-3 antibody
Anti-Igf-Binding Protein 3 antibody
Anti-Igfbp-3 antibody
Anti-IGFBP3 antibody
Anti-IBP3 antibody

Information sourced from Uniprot.org

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