• Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-IGF2 antibody (25-40 aa) (STJ191865)

SKU:
STJ191865

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Insulin-like growth factor II (25-40 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IGF2
Gene ID: 3481
Uniprot ID: IGF2_HUMAN
Immunogen Region: 25-40 aa
Specificity: IGF2 Polyclonal Antibody detects endogenous levels of protein.
Immunogen: Synthesized peptide derived from part of the human protein at the amino acid range 25-40
Post Translational Modifications O-glycosylated with core 1 or possibly core 8 glycans. Thr-96 is a minor glycosylation site compared to Thr-99. Proteolytically processed by PCSK4, proIGF2 is cleaved at Arg-128 and Arg-92 to generate big-IGF2 and mature IGF2.
Function The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF2 is influenced by placental lactogen. Also involved in tissue differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver (Probable). Acts as a ligand for integrin which is required for IGF2 signaling. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation. Inhibits myoblast differentiation and modulates metabolism via increasing the mitochondrial respiration rate. Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
Protein Name Insulin-Like Growth Factor Ii
Igf-Ii
Somatomedin-A
T3m-11-Derived Growth Factor Cleaved Into - Insulin-Like Growth Factor Ii - Insulin-Like Growth Factor Ii Ala-25 Del - Preptin
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-2404192
Reactome: R-HSA-2428928
Reactome: R-HSA-2428933
Reactome: R-HSA-381426
Cellular Localisation Secreted
Alternative Antibody Names Anti-Insulin-Like Growth Factor Ii antibody
Anti-Igf-Ii antibody
Anti-Somatomedin-A antibody
Anti-T3m-11-Derived Growth Factor Cleaved Into - Insulin-Like Growth Factor Ii - Insulin-Like Growth Factor Ii Ala-25 Del - Preptin antibody
Anti-IGF2 antibody
Anti-PP1446 antibody

Information sourced from Uniprot.org