• Western blot analysis of various lysates using IGF1 Rabbit polyclonal antibody (STJ11105441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-IGF1 antibody (65-165) (STJ11105441)

SKU:
STJ11105441

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Insulin-Like Growth Factor I (65-165) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IGF1
Gene ID: 3479
Uniprot ID: IGF1_HUMAN
Immunogen Region: 65-165
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 65-165 of human IGF1 (NP_000609.1).
Immunogen Sequence: VCGDRGFYFNKPTGYGSSSR RAPQTGIVDECCFRSCDLRR LEMYCAPLKPAKSARSVRAQ RHTDMPKTQKYQPPSTNKNT KSQRRKGWPKTHPGGEQKEG T
Function The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of 1-14C-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.
Protein Name Insulin-Like Growth Factor I
Igf-I
Mechano Growth Factor
Mgf
Somatomedin-C
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-2404192
Reactome: R-HSA-2428928
Reactome: R-HSA-2428933
Reactome: R-HSA-381426
Reactome: R-HSA-422085
Cellular Localisation Secreted
Alternative Antibody Names Anti-Insulin-Like Growth Factor I antibody
Anti-Igf-I antibody
Anti-Mechano Growth Factor antibody
Anti-Mgf antibody
Anti-Somatomedin-C antibody
Anti-IGF1 antibody
Anti-IBP1 antibody

Information sourced from Uniprot.org

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