Anti-IGF1 antibody (50-150) (STJ113891)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal IGF1 (50-150) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1500-1:3000IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | IGF1 |
| Gene ID | 3479 |
| Uniprot ID | IGF1_HUMAN |
| Immunogen Region | 50-150 |
| Immunogen Sequence | PETLCGAELVDALQFVCGDR GFYFNKPTGYGSSSRRAPQT GIVDECCFRSCDLRRLEMYC APLKPAKSARSVRAQRHTDM PKTQKEVHLKNASRGSAGNK N |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human IGF1 (NP_000609.1). |
Additional Info
| Function | The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of 1-14C-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM. |
| Protein Name | Insulin-Like Growth Factor 1Insulin-Like Growth Factor IIgf-IMechano Growth FactorMgfSomatomedin-C |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-2404192Reactome: R-HSA-2428928Reactome: R-HSA-2428933Reactome: R-HSA-381426Reactome: R-HSA-422085 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Insulin-Like Growth Factor 1 antibodyAnti-Insulin-Like Growth Factor I antibodyAnti-Igf-I antibodyAnti-Mechano Growth Factor antibodyAnti-Mgf antibodyAnti-Somatomedin-C antibodyAnti-IGF1 antibodyAnti-IBP1 antibodyAnti-IGF-1 antibody |
Information sourced from Uniprot.org