| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Intraflagellar transport protein 20 homolog (62-112 aa) is suitable for use in Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:1000-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | IFT20 |
| Gene ID: | 90410 |
| Uniprot ID: | IFT20_HUMAN |
| Immunogen Region: | 62-112 aa |
| Specificity: | This antibody detects endogenous levels of human IFT20 |
| Immunogen: | Synthesized peptide derived from the human IFT20 at the amino acid range 62-112 |
| Function | Part of intraflagellar transport (IFT) particles involved in ciliary process assembly. May play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium. Regulates the platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway. Required for protein stability of E3 ubiquitin ligases CBL and CBLB that mediate ubiquitination and internalization of PDGFRA for proper feedback inhibition of PDGFRA signaling. Essential for male fertility. Plays an important role in spermatogenesis, particularly spermiogenesis, when germ cells form flagella. May play a role in the transport of flagellar proteins ODF2 and SPAG16 to build sperm flagella and in the removal of redundant sperm cytoplasm. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment. |
| Protein Name | Intraflagellar Transport Protein 20 Homolog Hift20 |
| Database Links | Reactome: R-HSA-5620924 |
| Cellular Localisation | Golgi Apparatus Cis-Golgi Network Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Centriole Cilium Basal Body Cell Projection Cilium Cytoplasmic Vesicle Secretory Vesicle Acrosome Present At The Centrosomes During The Cell Cycle And Associated With The Proximal Portion Of The Mother Centriole And The Lateral Aspect Of The Daughter Centriole Associated With Basal Body At The Base Of Primary Cilia Detected In The Golgi Apparatus Of Round Spermatids And Late Spermatocytes Also Detected In The Manchette Of Step 10-12 Spermatids In Step 14 Spermatids Found In The Basal Body Of The Sperm Tail Localization In The Manchette Of Elongating Spermatids Is Dependent On Spag17 |
| Alternative Antibody Names | Anti-Intraflagellar Transport Protein 20 Homolog antibody Anti-Hift20 antibody Anti-IFT20 antibody |
Information sourced from Uniprot.org