![Anti-IFNG/IFN-gamma nanobody [SAA1294] (STJN000082)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/200695/412877/STJN000082-SDS-PAGE-1__85605.1749232420.jpg?c=1 "Anti-IFNG/IFN-gamma nanobody [SAA1294] (STJN000082)")
![Anti-IFNG/IFN-gamma nanobody [SAA1294] (STJN000082)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/200695/412877/STJN000082-SDS-PAGE-1__85605.1749232420.jpg?c=1 "Anti-IFNG/IFN-gamma nanobody [SAA1294] (STJN000082)")
Anti-IFNG/IFN-gamma nanobody [SAA1294] (STJN000082)
SPECIFICATIONS
ClonalityMonoclonal
HostAlpaca
ConjugationUnconjugated
IsotypeVHH-8His-Cys-tag
General Information
| Short Description | Alpaca monoclonal nanobody anti-Interferon Gamma is suitable for use in ELISA research applications. |
| Applications | ELISA |
| Host | Alpaca |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SAA1294 |
| Isotype | VHH-8His-Cys-tag |
| Conjugation | Unconjugated |
| Concentration | 0.51 mg/mL |
| Purification | Purified by Nickel column. |
| Formulation | 0.01M PBS, pH 7.4. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at-20°C 12 months. Store at-80°C long term. |
Target Information
| Gene Symbol | IFNG |
| Gene ID | 3458 |
| Uniprot ID | IFNG_HUMAN |
Additional Info
| Post Translational Modifications | Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. |
| Function | Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation. |
| Protein Name | Interferon GammaIfn-GammaImmune Interferon |
| Database Links | Reactome: R-HSA-877300Reactome: R-HSA-877312Reactome: R-HSA-8877330Reactome: R-HSA-8950505Reactome: R-HSA-9732724 |
| Cellular Localisation | Secreted |
| Alternative Nanobody Names | Anti-Interferon Gamma nanobodyAnti-Ifn-Gamma nanobodyAnti-Immune Interferon nanobodyAnti-IFNG nanobody |
Information sourced from Uniprot.org