• Western blot analysis of extracts of various cell lines, using IER3 antibody (STJ111301) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human gastric cancer using IER3 antibody (STJ111301) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse stomach using IER3 antibody (STJ111301) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse stomach using IER3 antibody (STJ111301) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human gastric cancer using IER3 antibody (STJ111301) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using IER3 antibody (STJ111301) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-IER3 antibody (50-150) (STJ111301)

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STJ111301

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IER3 (50-150) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IER3
Gene ID: 8870
Uniprot ID: IEX1_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human IER3 (NP_003888.2).
Immunogen Sequence: GRPSASRGHRKRSRRVLYPR VVRRQLPVEEPNPAKRLLFL LLTIVFCQILMAEEGVPAPL PPEDAPNAASLAPTPVSAVL EPFNLTSEPSDYALDLSTFL Q
Post Translational Modifications Phosphorylated at Thr-18, Thr-123 and Ser-126 by MAPK1/ERK2 and probably MAPK3/ERK1. Upon phosphorylation by MAPK1/ERK2 and MAPK3/ERK1, acquires the ability to inhibit cell death induced by various stimuli. Glycosylated.
Function May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment.
Protein Name Radiation-Inducible Immediate-Early Gene Iex-1
Differentiation-Dependent Gene 2 Protein
Protein Dif-2
Immediate Early Protein Gly96
Immediate Early Response 3 Protein
Pacap-Responsive Gene 1 Protein
Protein Prg1
Database Links Reactome: R-HSA-6811558
Cellular Localisation Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Radiation-Inducible Immediate-Early Gene Iex-1 antibody
Anti-Differentiation-Dependent Gene 2 Protein antibody
Anti-Protein Dif-2 antibody
Anti-Immediate Early Protein Gly96 antibody
Anti-Immediate Early Response 3 Protein antibody
Anti-Pacap-Responsive Gene 1 Protein antibody
Anti-Protein Prg1 antibody
Anti-IER3 antibody
Anti-DIF2 antibody
Anti-IEX1 antibody
Anti-PRG1 antibody

Information sourced from Uniprot.org

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