Anti-ICK antibody (1-270) (STJ110093)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ICK (1-270) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CILK1 |
| Gene ID | 22858 |
| Uniprot ID | CILK1_HUMAN |
| Immunogen Region | 1-270 |
| Immunogen Sequence | MNRYTTIRQLGDGTYGSVLL GRSIESGELIAIKKMKRKFY SWEECMNLREVKSLKKLNHA NVVKLKEVIRENDHLYFIFE YMKENLYQLIKERNKLFPES AIRNIMYQILQGLAFIHKHG FFHRDLKPENLLCMGPELVK IADFGLAREIRSKPPYTDYV STRWYRAPEVLLRSTNYSSP IDVWAVGCIMAEVYTLRPLF PGASEIDTIFKICQVLGTPK KTDWPEGYQLSSAMNFRWP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ICK (NP_057597.2). |
Additional Info
| Tissue Specificity | Expressed in heart, brain, placenta, pancreas, thymus, prostate, testis, ovary, small intestine and colon, with highest levels in placenta and testis. Not detected in spleen. Also expressed in many cancer cell lines. |
| Post Translational Modifications | Autophosphorylated on serine and threonine residues. Phosphorylation at Thr-157 increases kinase activity. |
| Function | Required for ciliogenesis. Phosphorylates KIF3A. Involved in the control of ciliary length. Regulates the ciliary localization of SHH pathway components as well as the localization of IFT components at ciliary tips. May play a key role in the development of multiple organ systems and particularly in cardiac development. Regulates intraflagellar transport (IFT) speed and negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner and this regulation requires its kinase activity. |
| Protein Name | Serine/Threonine-Protein Kinase IckCiliogenesis Associated Kinase 1Intestinal Cell KinaseHickLaryngeal Cancer Kinase 2Lck2Mak-Related KinaseMrk |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmCytosolCell ProjectionCiliumCytoskeletonCilium Basal BodyAlso Found At The Ciliary TipNuclear Localization Has Been Observed With A Gfp-Tagged Construct In Transfected Hela CellsIsoform 2: CytoplasmPredominant Cytoplasmic Localization Has Been Observed With A N-Terminally Gfp-Tagged Construct |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Ick antibodyAnti-Ciliogenesis Associated Kinase 1 antibodyAnti-Intestinal Cell Kinase antibodyAnti-Hick antibodyAnti-Laryngeal Cancer Kinase 2 antibodyAnti-Lck2 antibodyAnti-Mak-Related Kinase antibodyAnti-Mrk antibodyAnti-CILK1 antibodyAnti-ICK antibodyAnti-KIAA0936 antibody |
Information sourced from Uniprot.org