• Western blot analysis of extracts of various cell lines, using IARS antibody (STJ112228) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-IARS antibody (1158-1262) (STJ112228)

SKU:
STJ112228

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IARS (1158-1262) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IARS1
Gene ID: 3376
Uniprot ID: SYIC_HUMAN
Immunogen Region: 1158-1262
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1158-1262 of human IARS (NP_002152.2).
Immunogen Sequence: SAPSLINSSSTLLCQYINLQ LLNAKPQECLMGTVGTLLLE NPLGQNGLTHQGLLYEAAKV FGLRSRKLKLFLNETQTQEI TEDIPVKTLNMKTVYVSVLP TTADF
Tissue Specificity Expressed in liver and muscle (at protein level).
Function Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
Protein Name Isoleucine--Trna Ligase - Cytoplasmic
Isoleucyl-Trna Synthetase
Irs
Ilers
Database Links Reactome: R-HSA-2408522
Reactome: R-HSA-379716
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Isoleucine--Trna Ligase - Cytoplasmic antibody
Anti-Isoleucyl-Trna Synthetase antibody
Anti-Irs antibody
Anti-Ilers antibody
Anti-IARS1 antibody
Anti-IARS antibody

Information sourced from Uniprot.org

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