• Immunohistochemistry analysis of paraffin-embedded rat pancreas using ORP150/GRP170/HSP12A Rabbit monoclonal antibody (STJ11101270) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ORP150/GRP170/HSP12A Rabbit monoclonal antibody (STJ11101270) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HYOU1 antibody (1-150) [S0MR] (STJ11101270)

SKU:
STJ11101270

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ORP150 (1-150) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HYOU1
Gene ID: 10525
Uniprot ID: HYOU1_HUMAN
Immunogen Region: 1-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human ORP150/GRP170/HSP12A (Q9Y4L1).
Immunogen Sequence: MADKVRRQRPRRRVCWALVA VLLADLLALSDTLAVMSVDL GSESMKVAIVKPGVPMEIVL NKESRRKTPVIVTLKENERF FGDSAASMAIKNPKATLRYF QHLLGKQADNPHVALYQARF PEHELTFDPQRQTVHFQISS QLQFSPEEVL
Tissue Specificity Highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers.
Function Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.
Protein Name Hypoxia Up-Regulated Protein 1
150 Kda Oxygen-Regulated Protein
Orp-150
170 Kda Glucose-Regulated Protein
Grp-170
Database Links Reactome: R-HSA-3000484
Reactome: R-HSA-381038
Cellular Localisation Endoplasmic Reticulum Lumen
Alternative Antibody Names Anti-Hypoxia Up-Regulated Protein 1 antibody
Anti-150 Kda Oxygen-Regulated Protein antibody
Anti-Orp-150 antibody
Anti-170 Kda Glucose-Regulated Protein antibody
Anti-Grp-170 antibody
Anti-HYOU1 antibody
Anti-GRP170 antibody
Anti-ORP150 antibody

Information sourced from Uniprot.org

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