Anti-HtrA2 antibody [R06-9E4] (STJA0034447)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human HtrA2/Omi
General Information
| Short Description | Rabbit monoclonal anti-HtrA2 for use in WB, IHC-F, IHC-P, ICC, IF and IP in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P/ICC/IF/IP |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R06-9E4 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | HTRA2 |
| Gene ID | 27429 |
| Uniprot ID | HTRA2_HUMAN |
| Immunogen | A synthetic peptide of human HtrA2/Omi |
Additional Info
| Tissue Specificity | Isoform 1: Ubiquitously expressed. |
| Post Translational Modifications | Ubiquitinated by BIRC6.this activity is inhibited by DIABLO/SMAC. Autoproteolytically activated. |
| Function | Isoform 1: Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes apoptosis by either relieving the inhibition of BIRC proteins on caspases, leading to an increase in caspase activity.or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves BIRC6 and relieves its inhibition on CASP3, CASP7 and CASP9, but it is also prone to inhibition by BIRC6. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2: Seems to be proteolytically inactive. |
| Protein Name | Serine Protease Htra2 - MitochondrialHigh Temperature Requirement Protein A2Htra2Omi Stress-Regulated EndoproteaseSerine Protease 25Serine Proteinase Omi |
| Database Links | Reactome: R-HSA-9837999Reactome: R-HSA-9841251 |
| Cellular Localisation | Mitochondrion Intermembrane SpaceMitochondrion MembraneSingle-Pass Membrane ProteinPredominantly Present In The Intermembrane SpaceReleased Into The Cytosol Following Apoptotic StimuliSuch As Uv TreatmentAnd Stimulation Of Mitochondria With Caspase-8 Truncated Bid/TbidIsoform 1: Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Serine Protease Htra2 - Mitochondrial antibodyAnti-High Temperature Requirement Protein A2 antibodyAnti-Htra2 antibodyAnti-Omi Stress-Regulated Endoprotease antibodyAnti-Serine Protease 25 antibodyAnti-Serine Proteinase Omi antibodyAnti-HTRA2 antibodyAnti-OMI antibodyAnti-PRSS25 antibody |
Information sourced from Uniprot.org