• Western blot analysis of extracts of Jurkat cells, using HTR4 antibody (STJ11103510) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using HTR4 antibody (STJ11103510) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HTR4 antibody (350-384) (STJ11103510)

SKU:
STJ11103510

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HTR4 (350-384) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HTR4
Gene ID: 3360
Uniprot ID: 5HT4R_HUMAN
Immunogen Region: 350-384
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 350-384 of human HTR4 (NP_000861.1).
Immunogen Sequence: TINGSTHVLRDAVECGGQWE SQCHPPATSPLVAAQ
Tissue Specificity Isoform 5-HT4(A) is expressed in ileum, brain, and atrium, but not in the ventricle.
Function This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.
Protein Name 5-Hydroxytryptamine Receptor 4
5-Ht-4
5-Ht4
Serotonin Receptor 4
Database Links Reactome: R-HSA-390666
Reactome: R-HSA-418555
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Endosome
Interaction With Snx27 Mediates Recruitment To Early Endosomes
While Interaction With Nherf1 And Ezr Might Target The Protein To Specialized Subcellular Regions
Such As Microvilli
Alternative Antibody Names Anti-5-Hydroxytryptamine Receptor 4 antibody
Anti-5-Ht-4 antibody
Anti-5-Ht4 antibody
Anti-Serotonin Receptor 4 antibody
Anti-HTR4 antibody

Information sourced from Uniprot.org

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