• Immunohistochemistry analysis of HTR3A in paraffin-embedded mouse brain using HTR3A Rabbit polyclonal antibody (STJ27614) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HTR3A in paraffin-embedded rat brain using HTR3A Rabbit polyclonal antibody (STJ27614) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HTR3A Rabbit polyclonal antibody (STJ27614) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-HTR3A antibody (24-484) (STJ27614)

SKU:
STJ27614

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HTR3A (24-484) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HTR3A
Gene ID: 3359
Uniprot ID: 5HT3A_HUMAN
Immunogen Region: 24-484
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-484 of human HTR3A (NP_000860.2).
Immunogen Sequence: LAQGEARRSRNTTRPALLRL SDYLLTNYRKGVRPVRDWRK PTTVSIDVIVYAILNVDEKN QVLTTYIWYRQYWTDEFLQW NPEDFDNITKLSIPTDSIWV PDILINEFVDVGKSPNIPYV YIRHQGEVQNYKPLQVVTAC SLDIYNFPFDVQNCSLTFTS WLHTIQDINISLWRLPEKVK SDRSVFMNQGEWELLGVLPY FREFSMESSNYYAEMKFY
Tissue Specificity Expressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta.
Function Forms serotonin (5-hydroxytryptamine/5-HT3)-activated cation-selective channel complexes, which when activated cause fast, depolarizing responses in neurons.
Protein Name 5-Hydroxytryptamine Receptor 3a
5-Ht3-A
5-Ht3a
5-Hydroxytryptamine Receptor 3
5-Ht-3
5-Ht3r
Serotonin Receptor 3a
Serotonin-Gated Ion Channel Receptor
Database Links Reactome: R-HSA-112314
Cellular Localisation Postsynaptic Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Alternative Antibody Names Anti-5-Hydroxytryptamine Receptor 3a antibody
Anti-5-Ht3-A antibody
Anti-5-Ht3a antibody
Anti-5-Hydroxytryptamine Receptor 3 antibody
Anti-5-Ht-3 antibody
Anti-5-Ht3r antibody
Anti-Serotonin Receptor 3a antibody
Anti-Serotonin-Gated Ion Channel Receptor antibody
Anti-HTR3A antibody
Anti-5HT3R antibody
Anti-HTR3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance