| Host: | Mouse |
| Applications: | WB/IHC/IF |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Heat shock protein beta-1 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality : | Monoclonal |
| Clone ID : | Mix-mA |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:1000-1:3000 IHC 1:50-1:200 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HSPB1 |
| Gene ID: | 3315 |
| Uniprot ID: | HSPB1_HUMAN |
| Specificity: | Antibody can detects endogenous HSP27 protein. |
| Immunogen: | Recombinant Protein of HSP27 |
| Post Translational Modifications | Phosphorylated upon exposure to protein kinase C activators and heat shock. Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement. |
| Function | Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. |
| Protein Name | Heat Shock Protein Beta-1 Hspb1 28 Kda Heat Shock Protein Estrogen-Regulated 24 Kda Protein Heat Shock 27 Kda Protein Hsp 27 Heat Shock Protein Family B Member 1 Stress-Responsive Protein 27 Srp27 |
| Database Links | Reactome: R-HSA-4420097 Reactome: R-HSA-450408 Reactome: R-HSA-5687128 Reactome: R-HSA-9009391 |
| Cellular Localisation | Cytoplasm Nucleus Cytoskeleton Spindle Cytoplasmic In Interphase Cells Colocalizes With Mitotic Spindles In Mitotic Cells Translocates To The Nucleus During Heat Shock And Resides In Sub-Nuclear Structures Known As Sc35 Speckles Or Nuclear Splicing Speckles |
| Alternative Antibody Names | Anti-Heat Shock Protein Beta-1 antibody Anti-Hspb1 antibody Anti-28 Kda Heat Shock Protein antibody Anti-Estrogen-Regulated 24 Kda Protein antibody Anti-Heat Shock 27 Kda Protein antibody Anti-Hsp 27 antibody Anti-Heat Shock Protein Family B Member 1 antibody Anti-Stress-Responsive Protein 27 antibody Anti-Srp27 antibody Anti-HSPB1 antibody Anti-HSP27 antibody Anti-HSP28 antibody |
Information sourced from Uniprot.org