• Immunofluorescence analysis of paraffin-embedded Mouse colon cancer tissue using Grp94 Rabbit polyclonal antibody (STJ24090) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of NIH/3T3 cells using Grp94 Rabbit polyclonal antibody (STJ24090) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Grp94 in paraffin-embedded mouse kidney using Grp94 Rabbit polyclonal antibody (STJ24090) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Grp94 in paraffin-embedded human lung cancer using Grp94 Rabbit polyclonal antibody (STJ24090) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Grp94 in paraffin-embedded rat testis using Grp94 Rabbit polyclonal antibody (STJ24090) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HSP90B1 antibody (40-310) (STJ24090)

SKU:
STJ24090

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HSP90B1 (40-310) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSP90B1
Gene ID: 7184
Uniprot ID: ENPL_HUMAN
Immunogen Region: 40-310
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 40-310 of human Grp94 (NP_003290.1).
Immunogen Sequence: EGSRTDDEVVQREEEAIQLD GLNASQIRELREKSEKFAFQ AEVNRMMKLIINSLYKNKEI FLRELISNASDALDKIRLIS LTDENALSGNEELTVKIKCD KEKNLLHVTDTGVGMTREEL VKNLGTIAKSGTSEFLNKMT EAQEDGQSTSELIGQFGVGF YSAFLVADKVIVTSKHNNDT QHIWESDSNEFSVIADPRGN TLGRGTTITLVLKEEASDYL ELDTIKNLVKKYSQFINFP
Function Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion.the translocation process is mediated by the cargo receptor TMED10.
Protein Name Endoplasmin
94 Kda Glucose-Regulated Protein
Grp-94
Heat Shock Protein 90 Kda Beta Member 1
Tumor Rejection Antigen 1
Gp96 Homolog
Database Links Reactome: R-HSA-1679131
Reactome: R-HSA-3000480
Reactome: R-HSA-381183
Reactome: R-HSA-381426
Reactome: R-HSA-6785807
Reactome: R-HSA-8957275
Cellular Localisation Endoplasmic Reticulum Lumen
Sarcoplasmic Reticulum Lumen
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Endoplasmin antibody
Anti-94 Kda Glucose-Regulated Protein antibody
Anti-Grp-94 antibody
Anti-Heat Shock Protein 90 Kda Beta Member 1 antibody
Anti-Tumor Rejection Antigen 1 antibody
Anti-Gp96 Homolog antibody
Anti-HSP90B1 antibody
Anti-GRP94 antibody
Anti-TRA1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance