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Anti-Hsp90 antibody [SM0475] (STJA0010475)

Western blot analysis of HSP90. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:3000, RT, 1h.
Immunohistochemistry analysis of HSP90. Sample: mouse testis, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
Immunohistochemistry analysis of HSP90. Sample: Rat testis, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
Immunohistochemistry analysis of HSP90. Sample: mouse ovary, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
Immunohistochemistry analysis of HSP90. Sample: mouse brain, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
Immunohistochemistry analysis of HSP90. Sample: Rat colon, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
Immunohistochemistry analysis of HSP90. Sample: Rat ovary, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
Immunohistochemistry analysis of HSP90. Sample: Rat brain, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
IF analysis of HSP90. Sample: mouse ovary, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:200 RT 1h.
IF analysis of HSP90. Sample: mouse testis, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:200 RT 1h.
IF analysis of HSP90. Sample: Rat ovary, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:200 RT 1h.
IF analysis of HSP90. Sample: Rat testis, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:200 RT 1h.

Anti-Hsp90 antibody [SM0475] (STJA0010475)

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STJA0010475

$230.18 - $315.23

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Mouse
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Mouse monoclonal antibody anti-Hsp90 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Monoclonal
Clone ID:	SM0475
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol.
Purification:	Affinity purification
Dilution Range:	WB (H, M, R) 1:500-1:1000 IHC/IF (M, R) 1:500-1:1000/1:500-1:1000
Storage Instruction:	Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.

Gene Symbol:	Hsp90aa1
Gene ID:	15519
Uniprot ID:	HS90A_MOUSE
Immunogen:	KLH conjugated Synthetic peptide corresponding to Mouse HSP90A

Function	Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle. Plays a critical role in mitochondrial import, delivers preproteins to the mitochondrial import receptor TOMM70. Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation. Mediates the association of TOMM70 with IRF3 or TBK1 in mitochondrial outer membrane which promotes host antiviral response.
Protein Name	Heat Shock Protein Hsp 90-Alpha Heat Shock 86 Kda Hsp 86 Hsp86 Tumor-Specific Transplantation 86 Kda Antigen Tsta
Database Links	Reactome: R-MMU-1227986 Reactome: -MMU-1474151 Reactome: -MMU-168928 Reactome: -MMU-2029482 Reactome: -MMU-203615 Reactome: -MMU-2565942 Reactome: -MMU-3371497 Reactome: -MMU-3371511 Reactome: -MMU-3371568 Reactome: -MMU-3371571 Reactome: -MMU-380259 Reactome: -MMU-380270 Reactome: -MMU-380284 Reactome: -MMU-380320 Reactome: -MMU-399954 Reactome: -MMU-4420097 Reactome: -MMU-5218920 Reactome: -MMU-5620912 Reactome: -MMU-5675482 Reactome: -MMU-6798695 Reactome: -MMU-8852276 Reactome: -MMU-8854518 Reactome: -MMU-8863795 Reactome: -MMU-8939211 Reactome: -MMU-9009391 Reactome: -MMU-9013418 Reactome: -MMU-9018519 Reactome: -MMU-9652282
Cellular Localisation	Nucleus Cytoplasm Melanosome Cell Membrane Mitochondrion
Alternative Antibody Names	Anti-Heat Shock Protein Hsp 90-Alpha antibody Anti-Heat Shock 86 Kda antibody Anti-Hsp 86 antibody Anti-Hsp86 antibody Anti-Tumor-Specific Transplantation 86 Kda Antigen antibody Anti-Tsta antibody Anti-Hsp90aa1 antibody Anti-Hsp86 antibody Anti-Hsp86-1 antibody Anti-Hspca antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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