• Immunohistochemistry analysis of paraffin-embedded human breast cancer using HRAS antibody (STJ11101732) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using HRAS antibody (STJ11101732) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HRAS antibody (90-189) [S2MR] (STJ11101732)

SKU:
STJ11101732

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-GTPase HRAS (90-189) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HRAS
Gene ID: 3265
Uniprot ID: RASH_HUMAN
Immunogen Region: 90-189
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 90-189 of human HRAS (P01112).
Immunogen Sequence: FEDIHQYREQIKRVKDSDDV PMVLVGNKCDLAARTVESRQ AQDLARSYGIPYIETSAKTR QGVEDAFYTLVREIRQHKLR KLNPPDESGPGCMSCKCVLS
Tissue Specificity Widely expressed.
Post Translational Modifications Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. S-nitrosylated.critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation. The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation. Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). Fatty-acylated at Lys-170. Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes. (Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL. Monoglucosylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to inhibit Ras signaling.
Function Involved in the activation of Ras protein signal transduction. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Protein Name Gtpase Hras
H-Ras-1
Ha-Ras
Transforming Protein P21
C-H-Ras
P21ras Cleaved Into - Gtpase Hras - N-Terminally Processed
Database Links Reactome: R-HSA-112412
Reactome: R-HSA-1169092
Reactome: R-HSA-1236382
Reactome: R-HSA-1250196
Reactome: R-HSA-1250347
Reactome: R-HSA-1433557
Reactome: R-HSA-167044
Reactome: R-HSA-171007
Reactome: R-HSA-179812
Reactome: R-HSA-180336
Reactome: R-HSA-186763
Reactome: R-HSA-1963640
Reactome: R-HSA-210993
Reactome: R-HSA-2179392
Reactome: R-HSA-2424491
Reactome: R-HSA-2428933
Reactome: R-HSA-2871796
Reactome: R-HSA-375165
Reactome: R-HSA-3928662
Reactome: R-HSA-442982
Reactome: R-HSA-5218921
Reactome: R-HSA-5621575
Reactome: R-HSA-5637810
Reactome: R-HSA-5654688
Reactome: R-HSA-5654693
Reactome: R-HSA-5654699
Reactome: R-HSA-5654700
Reactome: R-HSA-5654704
Reactome: R-HSA-5654706
Reactome: R-HSA-5654712
Reactome: R-HSA-5654719
Reactome: R-HSA-5655253
Reactome: R-HSA-5655291
Reactome: R-HSA-5655302
Reactome: R-HSA-5655332
Reactome: R-HSA-5658442
Reactome: R-HSA-5673000
Reactome: R-HSA-5673001
Reactome: R-HSA-5674135
Reactome: R-HSA-5675221
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802953
Reactome: R-HSA-6802955
Reactome: R-HSA-74751
Reactome: R-HSA-8849471
Reactome: R-HSA-8851805
Reactome: R-HSA-9026519
Reactome: R-HSA-9027284
Reactome: R-HSA-9028731
Reactome: R-HSA-9034864
Reactome: R-HSA-9607240
Reactome: R-HSA-9634285
Reactome: R-HSA-9634635
Reactome: R-HSA-9648002
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9664565
Reactome: R-HSA-9665348
Reactome: R-HSA-9665686
Reactome: R-HSA-9670439
Reactome: R-HSA-9673767
Reactome: R-HSA-9673770
Reactome: R-HSA-9703465
Reactome: R-HSA-9703648
Reactome: R-HSA-9753510
Reactome: R-HSA-9753512
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Golgi Apparatus
Golgi Apparatus Membrane
The Active Gtp-Bound Form Is Localized Most Strongly To Membranes Than The Inactive Gdp-Bound Form
Shuttles Between The Plasma Membrane And The Golgi Apparatus
Isoform 2: Nucleus
Cytoplasm
Perinuclear Region
Colocalizes With Rack1 To The Perinuclear Region
Alternative Antibody Names Anti-Gtpase Hras antibody
Anti-H-Ras-1 antibody
Anti-Ha-Ras antibody
Anti-Transforming Protein P21 antibody
Anti-C-H-Ras antibody
Anti-P21ras Cleaved Into - Gtpase Hras - N-Terminally Processed antibody
Anti-HRAS antibody
Anti-HRAS1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance