• Confocal imaging of NIH/3T3 cells using 15-PGDH/HPGD Rabbit monoclonal antibody (STJ11102225, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of C6 cells using 15-PGDH/HPGD Rabbit monoclonal antibody (STJ11102225, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of various lysates using 15-PGDH/HPGD Rabbit monoclonal antibody (STJ11102225) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HPGD antibody (50-151) [S5MR] (STJ11102225)

SKU:
STJ11102225

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PGDH (50-151) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HPGD
Gene ID: 3248
Uniprot ID: PGDH_HUMAN
Immunogen Region: 50-151
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-151 of human 15-PGDH/HPGD (P15428).
Immunogen Sequence: DEQFEPQKTLFIQCDVADQQ QLRDTFRKVVDHFGRLDILV NNAGVNNEKNWEKTLQINLV SVISGTYLGLDYMSKQNGGE GGIIINMSSLAGLMPVAQQP VY
Tissue Specificity Detected in colon epithelium (at protein level).
Function Catalyzes the NAD-dependent dehydrogenation (oxidation) of a broad array of hydroxylated polyunsaturated fatty acids (mainly eicosanoids and docosanoids, including prostaglandins, lipoxins and resolvins), yielding their corresponding keto (oxo) metabolites. Decreases the levels of the pro-proliferative prostaglandins such as prostaglandin E2 (whose activity is increased in cancer because of an increase in the expression of cyclooxygenase 2) and generates oxo-fatty acid products that can profoundly influence cell function by abrogating pro-inflammatory cytokine expression. Converts resolvins E1, D1 and D2 to their oxo products, which represents a mode of resolvin inactivation. Resolvin E1 plays important roles during the resolution phase of acute inflammation, while resolvins D1 and D2 have a unique role in obesity-induced adipose inflammation.
Protein Name 15-Hydroxyprostaglandin Dehydrogenase Nad(+
15-Pgdh
Eicosanoid/Docosanoid Dehydrogenase Nad(+
Prostaglandin Dehydrogenase 1
Short Chain Dehydrogenase/Reductase Family 36c Member 1
Database Links Reactome: R-HSA-2142700
Reactome: R-HSA-2162123
Reactome: R-HSA-9018676
Reactome: R-HSA-9018896
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-15-Hydroxyprostaglandin Dehydrogenase Nad(+ antibody
Anti-15-Pgdh antibody
Anti-Eicosanoid/Docosanoid Dehydrogenase Nad(+ antibody
Anti-Prostaglandin Dehydrogenase 1 antibody
Anti-Short Chain Dehydrogenase/Reductase Family 36c Member 1 antibody
Anti-HPGD antibody
Anti-PGDH1 antibody
Anti-SDR36C1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance