• Western blot analysis of extracts of Rat lung, using HOXB5 antibody (STJ117848) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 2s.
  • Western blot analysis of extracts of various cell lines, using HOXB5 antibody (STJ117848) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of U2OS cells using HOXB5 antibody (STJ117848) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HOXB5 antibody (170-269) (STJ117848)

SKU:
STJ117848

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HOXB5 (170-269) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HOXB5
Gene ID: 3215
Uniprot ID: HXB5_HUMAN
Immunogen Region: 170-269
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 170-269 of human HOXB5 (NP_002138.1).
Immunogen Sequence: EGQTPQIFPWMRKLHISHDM TGPDGKRARTAYTRYQTLEL EKEFHFNRYLTRRRRIEIAH ALCLSERQIKIWFQNRRMKW KKDNKLKSMSLATAGSAFQP
Tissue Specificity Spinal cord.
Function Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Protein Name Homeobox Protein Hox-B5
Homeobox Protein Hho.c10
Homeobox Protein Hox-2a
Homeobox Protein Hu-1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Homeobox Protein Hox-B5 antibody
Anti-Homeobox Protein Hho.c10 antibody
Anti-Homeobox Protein Hox-2a antibody
Anti-Homeobox Protein Hu-1 antibody
Anti-HOXB5 antibody
Anti-HOX2A antibody

Information sourced from Uniprot.org

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