| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Mouse/Rat/Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-HOMER1 (Internal Region) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
Homer1 |
| Gene ID: |
26556 |
| Uniprot ID: |
HOME1_MOUSE |
| Immunogen Region: |
Internal Region |
| Specificity: |
Specific for Homer1. |
| Immunogen: |
A synthetic peptide from the internal region of human HOMER1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse, rat, monkey and xenopus. |
| Function | Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Differentially regulates the functions of the calcium activated channel ryanodine receptors RYR1 and RYR2. Isoform 1 decreases the activity of RYR2, and increases the activity of RYR1, whereas isoform 5 counteracts the effects by competing for binding sites. Isoform 3 regulates the trafficking and surface expression of GRM5. Isoform 5 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1, isoform 2 and isoform 3 to regulate synaptic metabotropic glutamate function. Isoform 5, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Forms a high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines. Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA. |
| Protein Name | Homer Protein Homolog 1Homer-1Vasp/Ena-Related Gene Up-Regulated During Seizure And Ltp 1Vesl-1 |
| Database Links | Reactome: R-MMU-6794361 |
| Cellular Localisation | CytoplasmPostsynaptic DensitySynapseCell ProjectionDendritic SpineIsoform 1 Inhibits Surface Expression Of Grm5 Causing It To Be Retained In The Endoplasmic ReticulumThe N-Terminal Of Isoform 2 May Facilitate Trafficking Of The Complex With Grm5 From The Endoplasmic Reticulum (Er) To The Plasma Membrane (Pm) |
| Alternative Antibody Names | Anti-Homer Protein Homolog 1 antibodyAnti-Homer-1 antibodyAnti-Vasp/Ena-Related Gene Up-Regulated During Seizure And Ltp 1 antibodyAnti-Vesl-1 antibodyAnti-Homer1 antibodyAnti-Vesl1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
