• STJ73399 (0. 3µg/ml) staining of MCF7 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ73399 (0. 1µg/ml) staining of NIH3T3 (A) , (0. 01ug/ml) nuclear NIH3T3 (B) lysate, + Peptide (C) (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-HNRNPA2B1 antibody (Internal) (STJ73399)

SKU:
STJ73399-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-HNRNPA2B1 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-5µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: HNRNPA2B1
Gene ID: 3181
Uniprot ID: ROA2_HUMAN
Immunogen Region: Internal
Accession Number: NP_002128.1; NP_112533.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_002128.1; NP_112533.1).
Immunogen Sequence: DTIEIITDRQS
Function Heterogeneous nuclear ribonucleoprotein (hnRNP) that associates with nascent pre-mRNAs, packaging them into hnRNP particles. The hnRNP particle arrangement on nascent hnRNA is non-random and sequence-dependent and serves to condense and stabilize the transcripts and minimize tangling and knotting. Packaging plays a role in various processes such as transcription, pre-mRNA processing, RNA nuclear export, subcellular location, mRNA translation and stability of mature mRNAs. Forms hnRNP particles with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleus. Involved in transport of specific mRNAs to the cytoplasm in oligodendrocytes and neurons: acts by specifically recognizing and binding the A2RE (21 nucleotide hnRNP A2 response element) or the A2RE11 (derivative 11 nucleotide oligonucleotide) sequence motifs present on some mRNAs, and promotes their transport to the cytoplasm. Specifically binds single-stranded telomeric DNA sequences, protecting telomeric DNA repeat against endonuclease digestion. Also binds other RNA molecules, such as primary miRNA (pri-miRNAs): acts as a nuclear 'reader' of the N6-methyladenosine (m6A) mark by specifically recognizing and binding a subset of nuclear m6A-containing pri-miRNAs. Binding to m6A-containing pri-miRNAs promotes pri-miRNA processing by enhancing binding of DGCR8 to pri-miRNA transcripts. Involved in miRNA sorting into exosomes following sumoylation, possibly by binding (m6A)-containing pre-miRNAs. Acts as a regulator of efficiency of mRNA splicing, possibly by binding to m6A-containing pre-mRNAs. Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform. Also plays a role in the activation of the innate immune response. Mechanistically, senses the presence of viral DNA in the nucleus, homodimerizes and is demethylated by JMJD6. In turn, translocates to the cytoplasm where it activates the TBK1-IRF3 pathway, leading to interferon alpha/beta production. (Microbial infection) Involved in the transport of HIV-1 genomic RNA out of the nucleus, to the microtubule organizing center (MTOC), and then from the MTOC to the cytoplasm: acts by specifically recognizing and binding the A2RE (21 nucleotide hnRNP A2 response element) sequence motifs present on HIV-1 genomic RNA, and promotes its transport.
Protein Name Heterogeneous Nuclear Ribonucleoproteins A2/B1
Hnrnp A2/B1
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-72203
Reactome: R-HSA-8950505
Cellular Localisation Nucleus
Nucleoplasm
Cytoplasm
Cytoplasmic Granule
Secreted
Extracellular Exosome
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Component Of Ribonucleosomes
Not Found In The Nucleolus
Found In Exosomes Following Sumoylation
Isoform A2: Nucleus
Predominantly Nucleoplasmic
However Is Also Found In The Cytoplasm Of Cells In Some Tissues
Alternative Antibody Names Anti-Heterogeneous Nuclear Ribonucleoproteins A2/B1 antibody
Anti-Hnrnp A2/B1 antibody
Anti-HNRNPA2B1 antibody
Anti-HNRPA2B1 antibody

Information sourced from Uniprot.org

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