Anti-hnRNP K antibody [R07-0A0] (STJA0034428)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human hnRNP K
General Information
| Short Description | Rabbit monoclonal anti-hnRNP K for use in WB, IHC-F, IHC-P, ICC, IF and IP in Human, Mouse, Rat and Hamster samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P/ICC/IF/IP |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat/Hamster |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R07-0A0 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | HNRNPK |
| Gene ID | 3190 |
| Uniprot ID | HNRPK_HUMAN |
| Immunogen | A synthetic peptide of human hnRNP K |
Additional Info
| Post Translational Modifications | Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine. Sumoylated by CBX4. Sumoylation is increased upon DNA damage, such as that produced by doxorubicin, etoposide, UV light and camptothecin, due to enhanced CBX4 phosphorylation by HIPK2 under these conditions. Ubiquitinated by MDM2. Doxorubicin treatment does not affect monoubiquitination, but slightly decreases HNRNPK poly-ubiquitination. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. |
| Function | One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction. As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. As part of a ribonucleoprotein complex composed at least of ZNF827, HNRNPL and the circular RNA circZNF827 that nucleates the complex on chromatin, may negatively regulate the transcription of genes involved in neuronal differentiation. |
| Protein Name | Heterogeneous Nuclear Ribonucleoprotein KHnrnp KTransformation Up-Regulated Nuclear ProteinTunp |
| Database Links | Reactome: R-HSA-4570464Reactome: R-HSA-72163Reactome: R-HSA-72203Reactome: R-HSA-9610379 |
| Cellular Localisation | CytoplasmNucleusNucleoplasmCell ProjectionPodosomeRecruited To P53/Tp53-Responsive PromotersIn The Presence Of Functional P53/Tp53In Case Of Asfv InfectionThere Is A Shift In The Localization Which Becomes Predominantly Nuclear |
| Alternative Antibody Names | Anti-Heterogeneous Nuclear Ribonucleoprotein K antibodyAnti-Hnrnp K antibodyAnti-Transformation Up-Regulated Nuclear Protein antibodyAnti-Tunp antibodyAnti-HNRNPK antibodyAnti-HNRPK antibody |
Information sourced from Uniprot.org