• Immunofluorescence analysis of HeLa cells using HMGN1 antibody (STJ27908) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using HMGN1 antibody (STJ27908) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.

Anti-HMGN1 antibody (1-100) (STJ27908)

SKU:
STJ27908

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HMGN1 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HMGN1
Gene ID: 3150
Uniprot ID: HMGN1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGN1 (NP_004956.5).
Immunogen Sequence: MPKRKVSSAEGAAKEEPKRR SARLSAKPPAKVEAKPKKAA AKDKSSDKKVQTKGKRGAKG KQAEVANQETKEDLPAENGE TKTEESPASDEAGEKEAKSD
Post Translational Modifications Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation. Phosphorylation favors cytoplasmic localization.
Function Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.
Protein Name Non-Histone Chromosomal Protein Hmg-14
High Mobility Group Nucleosome-Binding Domain-Containing Protein 1
Database Links Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Cellular Localisation Nucleus
Cytoplasm
Cytoplasmic Enrichment Upon Phosphorylation
The Rna Edited Version Localizes To The Nucleus
Alternative Antibody Names Anti-Non-Histone Chromosomal Protein Hmg-14 antibody
Anti-High Mobility Group Nucleosome-Binding Domain-Containing Protein 1 antibody
Anti-HMGN1 antibody
Anti-HMG14 antibody

Information sourced from Uniprot.org

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