• Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using HMGA1 antibody (STJ11102046) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Confocal imaging of U-2 OS cells using HMGA1 Rabbit monoclonal antibody (STJ11102046, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Immunohistochemistry analysis of HMGA1 in paraffin-embedded human oophoroma using HMGA1 Rabbit monoclonal antibody (STJ11102046) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HMGA1 in paraffin-embedded rat brain using HMGA1 Rabbit monoclonal antibody (STJ11102046) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse testis, using HMGA1 Rabbit monoclonal antibody (STJ11102046) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using HMGA1 Rabbit monoclonal antibody (STJ11102046) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Chromatin immunoprecipitation was performed with 25 Mu g of cross-linked chromatin from HepG2 cells using 5 Mu g of HMGA1 Rabbit monoclonal antibody (STJ11102046). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of HMGA1 in the representative genomic region surrounding HNF1A gene.
  • Chromatin immunoprecipitation was performed with 25 Mu g of cross-linked chromatin from HepG2 cells using 5 Mu g of HMGA1 Rabbit monoclonal antibody (STJ11102046). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of HMGA1 across chromosome 12 (upper panel) and the genomic region encompassing HNF1A, a representative gene enriched in HMGA1 (lower panel).

Anti-HMGA1 antibody (1-107) [S6MR] (STJ11102046)

SKU:
STJ11102046

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-HMGA1 (1-107) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
ChIP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HMGA1
Gene ID: 3159
Uniprot ID: HMGA1_HUMAN
Immunogen Region: 1-107
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-107 of human HMGA1 (P17096).
Immunogen Sequence: MSESSSKSSQPLASKQEKDG TEKRGRGRPRKQPPVSPGTA LVGSQKEPSEVPTPKRPRGR PKGSKNKGAAKTRKTTTTPG RKPRGRPKKLEKEEEEGISQ ESSEEEQ
Post Translational Modifications Constitutively phosphorylated on two or three sites. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoforms HMG-I and HMG-Y can be phosphorylated by HIPK2. Phosphorylation of HMG-I at Ser-36, Thr-53 and Thr-78 and of HMG-Y at Thr-42 and Thr-67 by HIPK2 modulates DNA-binding affinity. HMG-Y is not methylated. Methylation at Arg-58 is mutually exclusive with methylation at Arg-60.
Function HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
Protein Name High Mobility Group Protein Hmg-I/Hmg-Y
Hmg-I(Y
High Mobility Group At-Hook Protein 1
High Mobility Group Protein A1
High Mobility Group Protein R
Database Links Reactome: R-HSA-162592
Reactome: R-HSA-164843
Reactome: R-HSA-175567
Reactome: R-HSA-177539
Reactome: R-HSA-180689
Reactome: R-HSA-180910
Reactome: R-HSA-2559584
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-High Mobility Group Protein Hmg-I/Hmg-Y antibody
Anti-Hmg-I(Y antibody
Anti-High Mobility Group At-Hook Protein 1 antibody
Anti-High Mobility Group Protein A1 antibody
Anti-High Mobility Group Protein R antibody
Anti-HMGA1 antibody
Anti-HMGIY antibody

Information sourced from Uniprot.org

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