![Western blot analysis of lysates from wild type (WT) and HMG20B knockout (KO) 293T cells, using [KO Validated] HMG20B Rabbit polyclonal antibody (STJ11100827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89763/358238/STJ11100827_1__18600.1713122571.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HMG20B knockout (KO) 293T cells, using [KO Validated] HMG20B Rabbit polyclonal antibody (STJ11100827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and HMG20B knockout (KO) 293T cells, using [KO Validated] HMG20B Rabbit polyclonal antibody (STJ11100827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89763/358238/STJ11100827_1__18600.1713122571.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HMG20B knockout (KO) 293T cells, using [KO Validated] HMG20B Rabbit polyclonal antibody (STJ11100827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-HMG20B antibody (1-317) (STJ11100827)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal HMG20B (1-317) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HMG20B |
| Gene ID | 10362 |
| Uniprot ID | HM20B_HUMAN |
| Immunogen Region | 1-317 |
| Immunogen Sequence | MSHGPKQPGAAAAPAGGKAP GQHGGFVVTVKQERGEGPRA GEKGSHEEEPVKKRGWPKGK KRKKILPNGPKAPVTGYVRF LNERREQIRTRHPDLPFPEI TKMLGAEWSKLQPTEKQRYL DEAEREKQQYMKELRAYQQS EAYKMCTEKIQEKKIKKEDS SSGLMNTLLNGHKGGDCDGF STFDVPIFTEEFLDQNKARE AELRRLRKMNVAFEEQNAVL QRHTQSMSSARERLEQELA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human HMG20B (NP_006330.2). |
Additional Info
| Tissue Specificity | Ubiquitously expressed in adult tissues. |
| Function | Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters. |
| Protein Name | Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily E Member 1-RelatedSmarce1-Related ProteinBrca2-Associated Factor 35Hmg Box-Containing Protein 20bHmg Domain-Containing Protein 2Hmg Domain-Containing Protein Hmgx2Sox-Like Transcriptional FactorStructural Dna-Binding Protein Braf35 |
| Database Links | Reactome: R-HSA-3214815Reactome: R-HSA-9679191Reactome: R-HSA-983231 |
| Cellular Localisation | NucleusChromosomeLocalized To Condensed Chromosomes In Mitosis In Conjunction With Brca2 |
| Alternative Antibody Names | Anti-Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily E Member 1-Related antibodyAnti-Smarce1-Related Protein antibodyAnti-Brca2-Associated Factor 35 antibodyAnti-Hmg Box-Containing Protein 20b antibodyAnti-Hmg Domain-Containing Protein 2 antibodyAnti-Hmg Domain-Containing Protein Hmgx2 antibodyAnti-Sox-Like Transcriptional Factor antibodyAnti-Structural Dna-Binding Protein Braf35 antibodyAnti-HMG20B antibodyAnti-BRAF35 antibodyAnti-HMGX2 antibodyAnti-HMGXB2 antibodyAnti-SMARCE1R antibody |
Information sourced from Uniprot.org