• Western blot analysis of extracts of various cell lines, using HLTF antibody (STJ110651) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of various cell lines, using HLTF antibody (STJ110651) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-HLTF antibody (726-1009) (STJ110651)

SKU:
STJ110651

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HLTF (726-1009) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HLTF
Gene ID: 6596
Uniprot ID: HLTF_HUMAN
Immunogen Region: 726-1009
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 726-1009 of human HLTF (NP_620636.1).
Immunogen Sequence: VSSNGPSGNDTPEELRKKLI RKMKLILSSGSDEECAICLD SLTVPVITHCAHVFCKPCIC QVIQNEQPHAKCPLCRNDIH EDNLLECPPEELARDSEKKS DMEWTSSSKINALMHALTDL RKKNPNIKSLVVSQFTTFLS LIEIPLKASGFVFTRLDGSM AQKKRVESIQCFQNTEAGSP TIMLLSLKAGGVGLNLSAAS RVFLMDPAWNPAAEDQCFDR CHRLGQKQEVIITKFIVKD
Tissue Specificity Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
Function Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity.This activity may be required for transcriptional activation or repression of specific target promoters. These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.
Protein Name Helicase-Like Transcription Factor
Dna-Binding Protein/Plasminogen Activator Inhibitor 1 Regulator
Hip116
Ring Finger Protein 80
Ring-Type E3 Ubiquitin Transferase Hltf
Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 3
Sucrose Nonfermenting Protein 2-Like 3
Database Links Reactome: R-HSA-8866654
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Nucleoplasm
Nuclear Localization Is Stimulated By Progesterone
Alternative Antibody Names Anti-Helicase-Like Transcription Factor antibody
Anti-Dna-Binding Protein/Plasminogen Activator Inhibitor 1 Regulator antibody
Anti-Hip116 antibody
Anti-Ring Finger Protein 80 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Hltf antibody
Anti-Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 3 antibody
Anti-Sucrose Nonfermenting Protein 2-Like 3 antibody
Anti-HLTF antibody
Anti-HIP116A antibody
Anti-RNF80 antibody
Anti-SMARCA3 antibody
Anti-SNF2L3 antibody
Anti-ZBU1 antibody

Information sourced from Uniprot.org

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