• Western blot analysis of extracts of various cell lines, using HLA-DOA antibody (STJ29003) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s.

Anti-HLA-DOA antibody (26-135) (STJ29003)

SKU:
STJ29003

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HLA-DOA (26-135) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HLA-DOA
Gene ID: 3111
Uniprot ID: DOA_HUMAN
Immunogen Region: 26-135
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-135 of human HLA-DOA (NP_002110.1).
Immunogen Sequence: TKADHMGSYGPAFYQSYGAS GQFTHEFDEEQLFSVDLKKS EAVWRLPEFGDFARFDPQGG LAGIAAIKAHLDILVERSNR SRAINVPPRVTVLPKSRVEL GQPNILICIV
Function Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM.
Protein Name Hla Class Ii Histocompatibility Antigen - Do Alpha Chain
Mhc Dn-Alpha
Mhc Dz Alpha
Mhc Class Ii Antigen Doa
Database Links Reactome: R-HSA-2132295
Cellular Localisation Endosome Membrane
Single-Pass Type I Membrane Protein
Lysosome Membrane
Complexes With Hla-Dm Molecule During Intracellular Transport And In Endosomal/Lysosomal Compartments
Heterotetramerization Is Necessary To Exit The Er
Alternative Antibody Names Anti-Hla Class Ii Histocompatibility Antigen - Do Alpha Chain antibody
Anti-Mhc Dn-Alpha antibody
Anti-Mhc Dz Alpha antibody
Anti-Mhc Class Ii Antigen Doa antibody
Anti-HLA-DOA antibody
Anti-HLA-DNA antibody
Anti-HLA-DZA antibody

Information sourced from Uniprot.org

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