| Tissue Specificity | Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific. Isoform 4: Testis-specific. |
| Function | Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome. |
| Protein Name | Hexokinase-1Brain Form HexokinaseHexokinase Type IHk IHexokinase-A |
| Database Links | Reactome: R-HSA-446205Reactome: R-HSA-5619056Reactome: R-HSA-70171 |
| Cellular Localisation | Mitochondrion Outer MembranePeripheral Membrane ProteinCytoplasmCytosolThe Mitochondrial-Binding Peptide (Mbp) Region Promotes Association With The Mitochondrial Outer Membrane (Probable)Dissociates From The Mitochondrial Outer Membrane Following Inhibition By N-Acetyl-D-GlucosamineLeading To Relocation To The Cytosol |
| Alternative Antibody Names | Anti-Hexokinase-1 antibodyAnti-Brain Form Hexokinase antibodyAnti-Hexokinase Type I antibodyAnti-Hk I antibodyAnti-Hexokinase-A antibodyAnti-HK1 antibody |
Information sourced from Uniprot.org
![Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain cells using 3 Mu g [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239). Western blot was performed from the immunoprecipitate using HK1 (STJ11101239) at a dilution of 1:1000.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90152/359011/STJ11101239_1__47294.1713123403.jpg?c=1 "Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain cells using 3 Mu g [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239). Western blot was performed from the immunoprecipitate using HK1 (STJ11101239) at a dilution of 1:1000.")
![Confocal imaging of paraffin-embedded mouse kidney using [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90152/359012/STJ11101239_2__86073.1713123404.jpg?c=1 "Confocal imaging of paraffin-embedded mouse kidney using [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.")
![Confocal imaging of U-2 OS cells using [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90152/359013/STJ11101239_3__81206.1713123404.jpg?c=1 "Confocal imaging of U-2 OS cells using [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.")
![Immunohistochemistry analysis of HK1 in paraffin-embedded rat kidney using [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90152/359014/STJ11101239_4__79194.1713123405.jpg?c=1 "Immunohistochemistry analysis of HK1 in paraffin-embedded rat kidney using [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of HK1 in paraffin-embedded mouse kidney using [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90152/359015/STJ11101239_5__87017.1713123406.jpg?c=1 "Immunohistochemistry analysis of HK1 in paraffin-embedded mouse kidney using [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of various lysates using [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90152/359016/STJ11101239_6__36859.1713123406.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western blot analysis of lysates from wild type (WT) and HK1 knockout (KO) 293T cells, using [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90152/359017/STJ11101239_7__78046.1713123407.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HK1 knockout (KO) 293T cells, using [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain cells using 3 Mu g [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239). Western blot was performed from the immunoprecipitate using HK1 (STJ11101239) at a dilution of 1:1000.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/90152/359011/STJ11101239_1__47294.1713123403.jpg?c=1 "Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain cells using 3 Mu g [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239). Western blot was performed from the immunoprecipitate using HK1 (STJ11101239) at a dilution of 1:1000.")
