• Anti-Histone H3 antibody (36-136) (STJ23980)
  • Chromatin immunoprecipitation analysis of extracts of 293T cells, using Histone H3 antibody (STJ23980) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunoprecipitation analysis of 600 Mu g extracts of HeLa cells using 10ug Histone H3 antibody (STJ23980). Western blot was performed from the immunoprecipitate using Histone H3 antibody (STJ23980) at a dilution of 1:30000.
  • Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Immunofluorescence analysis of paraffin-embedded rat testis using Histone H3 Rabbit polyclonal antibody (STJ23980) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Immunofluorescence analysis of paraffin-embedded mouse testis using Histone H3 Rabbit polyclonal antibody (STJ23980) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Histone H3 in paraffin-embedded mouse kidney using Histone H3 Rabbit polyclonal antibody (STJ23980) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Histone H3 in paraffin-embedded human brain using Histone H3 Rabbit polyclonal antibody (STJ23980) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Histone H3 in paraffin-embedded human liver using Histone H3 Rabbit polyclonal antibody (STJ23980) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Histone H3 in paraffin-embedded rat lung using Histone H3 Rabbit polyclonal antibody (STJ23980) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Histone H3 Rabbit polyclonal antibody (STJ23980) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Histone H3 antibody (36-136) (STJ23980)

SKU:
STJ23980

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/IP/ELISA/ChIP
Reactivity: Human/Mouse/Rat/Other
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:2000-1:10000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your spec
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H3-4
H3C1.H3C2.H3C3.H3C4.H3C6.
Gene ID: 8290
8350/8351/8352/8353/8354/8355/8356/8357/8358/8968
Uniprot ID: H31T_HUMAN
H31_HUMAN
Immunogen Region: 36-136
Specificity: A synthetic peptide corresponding to a sequence within amino acids 36-136 of human HIST3H3 (NP_003520.1).
Immunogen Sequence: VKKPHRYRPGTVALREIRRY QKSTELLIRKLPFQRLVREI AQDFKTDLRFQSSAVMALQE ACEAYLVGLFEDTNLCAIHA KRVTIMPKDIQLARRIRGER A
Tissue Specificity
Alternative Antibody Names Anti-Histone H3.1t antibody
Anti-H3/T antibody
Anti-H3t antibody
Anti-H3/G antibody
Anti-Histone H3.4 antibody
Anti-H3-4 antibody
Anti-H3FT antibody
Anti-HIST3H3 antibody
Anti-Histone H3.1 antibody
Anti-Histone H3/A antibody
Anti-Histone H3/B antibody
Anti-Histone H3/C antibody
Anti-Histone H3/D antibody
Anti-Histone H3/F antibody
Anti-Histone H3/H antibody
Anti-Histone H3/I antibody
Anti-Histone H3/J antibody
Anti-Histone H3/K antibody
Anti-Histone H3/L antibody
Anti-H3C1 antibody
Anti-H3FA antibody
Anti-HIST1H3A.H3C2 antibody
Anti-H3FL antibody
Anti-HIST1H3B.H3C3 antibody
Anti-H3FC antibody
Anti-HIST1H3C.H3C4 antibody
Anti-H3FB antibody
Anti-HIST1H3D.H3C6 antibody
Anti-H3FD antibody
Anti-HIST1H3E.H3C7 antibody
Anti-H3FI antibody
Anti-HIST1H3F.H3C8 antibody
Anti-H3FH antibody
Anti-HIST1H3G.H3C10 antibody
Anti-H3FK antibody
Anti-HIST1H3H.H3C11 antibody
Anti-H3FF antibody
Anti-HIST1H3I.H3C12 antibody
Anti-H3FJ antibody
Anti-HIST1H3J antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance