• Immunohistochemistry analysis of Histone H2B in paraffin-embedded mouse liver using Histone H2B Rabbit polyclonal antibody (STJ23969) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Histone H2B in paraffin-embedded rat kidney using Histone H2B Rabbit polyclonal antibody (STJ23969) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Histone H2B in paraffin-embedded human esophagus using Histone H2B Rabbit polyclonal antibody (STJ23969) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using Histone H2B Rabbit polyclonal antibody (STJ23969) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Histone H2B antibody (1-126) (STJ23969)

SKU:
STJ23969

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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat/Other
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:2000-1:10000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H2BC4.H2BC6.H2BC7.H2BC8.H2BC10
H2BC21
Gene ID: 3017/8339/8343/8344/8346/8347
8349
Uniprot ID: H2B1C_HUMAN
H2B2E_HUMAN
Immunogen Region: 1-126
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Histone H2B (NP_003519.1).
Immunogen Sequence: MPEPAKSAPAPKKGSKKAVT KAQKKDGKKRKRSRKESYSI YVYKVLKQVHPDTGISSKAM GIMNSFVNDIFERIAGEASR LAHYNKRSTITSREIQTAVR
Tissue Specificity
Alternative Antibody Names Anti-Histone H2b Type 1-C/E/F/G/I antibody
Anti-Histone H2b.1 A antibody
Anti-Histone H2b.a antibody
Anti-H2b/A antibody
Anti-Histone H2b.g antibody
Anti-H2b/G antibody
Anti-Histone H2b.h antibody
Anti-H2b/H antibody
Anti-Histone H2b.k antibody
Anti-H2b/K antibody
Anti-Histone H2b.l antibody
Anti-H2b/L antibody
Anti-H2BC4 antibody
Anti-H2BFL antibody
Anti-HIST1H2BC.H2BC6 antibody
Anti-H2BFH antibody
Anti-HIST1H2BE.H2BC7 antibody
Anti-H2BFG antibody
Anti-HIST1H2BF.H2BC8 antibody
Anti-H2BFA antibody
Anti-HIST1H2BG.H2BC10 antibody
Anti-H2BFK antibody
Anti-HIST1H2BI antibody
Anti-Histone H2b Type 2-E antibody
Anti-H2b-Clustered Histone 21 antibody
Anti-Histone H2b-Gl105 antibody
Anti-Histone H2b.q antibody
Anti-H2b/Q antibody
Anti-H2BC21 antibody
Anti-H2BFQ antibody
Anti-HIST2H2BE antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance