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Anti-Histone H2A antibody (Internal) (STJ96865)

Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, Histone H2A Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of HeLa cells using Histone H2A Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Histone H2A Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Histone H2A antibody (Internal) (STJ96865)

SKU:

STJ96865

£46.00 - £226.00

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Histone H2A type 1 (Internal) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC-P 1:100-1:300 ELISA 1:20000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	H2AC11.H2AC13.H2AC15.H2AC16.H2AC17
Gene ID:	8329/8330/8332/8336/8969
Uniprot ID:	H2A1_HUMAN
Immunogen Region:	Internal
Specificity:	Histone H2A Polyclonal Antibody detects endogenous levels of Histone H2A protein.
Immunogen:	Synthesized peptide derived from the Internal region of human Histone H2A. 20-50

Function	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Protein Name	Histone H2a Type 1 H2a.1 Histone H2a/Ptl
Database Links	Reactome: R-HSA-3214815 Reactome: R-HSA-3214847 Reactome: R-HSA-3214858 Reactome: R-HSA-5689603 Reactome: R-HSA-5689880 Reactome: R-HSA-5689901 Reactome: R-HSA-9609690 Reactome: R-HSA-9610379
Cellular Localisation	Nucleus Chromosome
Alternative Antibody Names	Anti-Histone H2a Type 1 antibody Anti-H2a.1 antibody Anti-Histone H2a/Ptl antibody Anti-H2AC11 antibody Anti-H2AFP antibody Anti-HIST1H2AG.H2AC13 antibody Anti-H2AFC antibody Anti-HIST1H2AI.H2AC15 antibody Anti-H2AFD antibody Anti-HIST1H2AK.H2AC16 antibody Anti-H2AFI antibody Anti-HIST1H2AL.H2AC17 antibody Anti-H2AFN antibody Anti-HIST1H2AM antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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