• Western blot analysis of extracts of various cell lines, using Histone H2A rabbit monoclonal antibody (STJ11101928) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of Rat liver, using Histone H2A rabbit monoclonal antibody (STJ11101928) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunohistochemistry analysis of paraffin-embedded human brain using Histone H2A rabbit monoclonal antibody (STJ11101928) at dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast using Histone H2A rabbit monoclonal antibody (STJ11101928) at dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using Histone H2A rabbit monoclonal antibody (STJ11101928) at dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Histone H2A rabbit monoclonal antibody (STJ11101928) at dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using Histone H2A rabbit monoclonal antibody (STJ11101928) at dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat colon using Histone H2A rabbit monoclonal antibody (STJ11101928) at dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using Histone H2A rabbit monoclonal antibody (STJ11101928) at dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g Histone H2A antibody (STJ11101928). Western blot was performed from the immunoprecipitate using Histone H2A antibody (STJ11101928) at a dilution of 1:1000.

Anti-HIST1H2AE antibody (1-100) [S8MR] (STJ11101928)

SKU:
STJ11101928

£146.50 - £642.50
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Host: Rabbit
Applications: WB/IHC-P/IP/ELISA
Reactivity: Human/Mouse/Rat/Other
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
IHC-P:1:200-1:2000
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requiremen
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H2AC4.H2AC8
H2AC11.H2AC13.H2AC15.H2AC16.H
Gene ID: 3012/8335
8329/8330/8332/8336/8969
Uniprot ID: H2A1B_HUMAN
H2A1_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H2A (P04908).
Immunogen Sequence: MSGRGKQGGKARAKAKTRSS RAGLQFPVGRVHRLLRKGNY SERVGAGAPVYLAAVLEYLT AEILELAGNAARDNKKTRII PRHLQLAIRNDEELNKLLGR
Tissue Specificity
Alternative Antibody Names Anti-Histone H2a Type 1-B/E antibody
Anti-Histone H2a.2 antibody
Anti-Histone H2a/A antibody
Anti-Histone H2a/M antibody
Anti-H2AC4 antibody
Anti-H2AFM antibody
Anti-HIST1H2AB.H2AC8 antibody
Anti-H2AFA antibody
Anti-HIST1H2AE antibody
Anti-Histone H2a Type 1 antibody
Anti-H2a.1 antibody
Anti-Histone H2a/Ptl antibody
Anti-H2AC11 antibody
Anti-H2AFP antibody
Anti-HIST1H2AG.H2AC13 antibody
Anti-H2AFC antibody
Anti-HIST1H2AI.H2AC15 antibody
Anti-H2AFD antibody
Anti-HIST1H2AK.H2AC16 antibody
Anti-H2AFI antibody
Anti-HIST1H2AL.H2AC17 antibody
Anti-H2AFN antibody
Anti-HIST1H2AM antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance