• Western blot analysis of extracts of normal 293T cells and 293T transfected with His-CEND1 protein, using His-Tag antibody (STJ11103695) at 1:100000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.
  • Western blot analysis of various lysates, using His-Tag monoclonal antibody (STJ11103695) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of normal 293T cells and 293T transfected with EPCAM protein, using His-Tag antibody (STJ11103695) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of 293T cells using mouse anti His-Tag monoclonal antibody (STJ11103695) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation of over-expressed His-tagged protein in 293T cells incubated using His-tag antibody (STJ11103695). A mock served as negative control and over-expressed 293T cell lysate served as positive control.
  • Immunoprecipitation analysis of 300ug extracts of 293T transfected with EPCAM Protein cells using 3ug His-Tag antibody (STJ11103695). Western blot was performed from the immunoprecipitate using His-Tag antibody (STJ11103695) at a dilition of 1:5000.

Anti-His-Tag antibody [S5MM] (STJ11103695)

SKU:
STJ11103695

£59.50 - £249.50
Current Stock:
Host: Mouse
Applications: WB/IF/ICC/IP/ELISA
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S5MM
Conjugation: Unconjugated
Isotype: IgG1
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:5000-1:20000
IF/ICC:1:50-1:200
IP:0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: A synthetic peptide corresponding to His tag.
Immunogen Sequence: HHHHHH
Background Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance