| Host: | Mouse |
| Applications: | WB/IF/ICC/IP/ELISA |
| Reactivity: | Species independent |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S5MM |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:5000-1:20000IF/ICC:1:50-1:200IP:0.5ug-4ug antibody for 200ug-400ug extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Specificity: | A synthetic peptide corresponding to His tag. |
| Immunogen Sequence: | HHHHHH |
| Background | Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies. |
Information sourced from Uniprot.org