What is His-Tag
The polyhistidine tag, also known as His-Tag is the most common affinity tag used for the purification of recombinant proteins. It consists of 6 to 10 consecutive histidines located at one of the termini of the target protein and is frequently separated by a protease-cleavage site.
His-Tag structure
Because of its small size (6-10 amino acids), the His-Tag is usually left on the recombinant protein and doesn't need to be removed, as it has little to no effect on the structure of the protein.
His-Tag Monoclonal antibodies
![Mouse monoclonal antibody anti-His-Tag](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/760x760/products/164638/322276/STJ11103695_3__55659.1650378804.jpg?c=1)
Mouse monoclonal antibody anti-His-Tag is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation.
His-Tag Polyclonal antibodies
![Rabbit polyclonal antibody anti-His-Tag](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/760x760/products/164650/322311/STJ11103707_1__71657.1650378824.jpg?c=1)
Rabbit polyclonal antibody anti-His-Tag is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation.
![Rabbit polyclonal antibody anti-His-Tag](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/760x760/products/112260/207123/STJ90106_2_WB__21339.1602845515.jpg?c=1)
Rabbit polyclonal antibody anti-His-Tag is suitable for use in Western Blot and ELISA research applications.