| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HIRA |
| Gene ID: | 7290 |
| Uniprot ID: | HIRA_HUMAN |
| Immunogen Region: | 250-550 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-550 of human HIRA (NP_003316.3). |
| Immunogen Sequence: | AQIIEREGWKTNMDFVGHRK AVTVVKFNPKIFKKKQKNGS SAKPSCPYCCCAVGSKDRSL SVWLTCLKRPLVVIHELFDK SIMDISWTLNGLGILVCSMD GSVAFLDFSQDELGDPLSEE EKSRIHQSTYGKSLAIMTEA QLSTAVIENPEMLKYQRRQQ QQQLDQKSAATREMGSATSV AGVVNGESLEDIRKNLLKKQ VETRTADGRRRITPLCIAQL DTGDFSTAFFNSIPLSGSL |
| Tissue Specificity | Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta. |
| Post Translational Modifications | Sumoylated. Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo. |
| Function | Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. |
| Protein Name | Protein HiraTup1-Like Enhancer Of Split Protein 1 |
| Database Links | Reactome: R-HSA-2559584Reactome: R-HSA-9821993 |
| Cellular Localisation | NucleusPml BodyPrimarilyThough Not ExclusivelyLocalized To The NucleusLocalizes To Pml Bodies Immediately Prior To Onset Of Senescence |
| Alternative Antibody Names | Anti-Protein Hira antibodyAnti-Tup1-Like Enhancer Of Split Protein 1 antibodyAnti-HIRA antibodyAnti-DGCR1 antibodyAnti-HIR antibodyAnti-TUPLE1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of various lysates using [KO Validated] HIRA Rabbit pAb (STJ110759) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/88962/405419/STJ110759_1__63725.1744891175.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] HIRA Rabbit pAb (STJ110759) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.")
