Anti-HINT1 antibody (5-18 aa) [R08-0E8] (STJA0012408)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human HINT1
General Information
| Short Description | Rabbit monoclonal anti-HINT1 (5-18 aa) for use in WB, ICC, IF and IP in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF/IP |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R08-0E8 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IF 1:50-1:200IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4ยฐC short term. Aliquot and store at-20ยฐC long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | HINT1 |
| Gene ID | 3094 |
| Uniprot ID | HINT1_HUMAN |
| Immunogen | A synthetic peptide of human HINT1 |
| Immunogen Region | 5-18 aa |
Additional Info
| Tissue Specificity | Widely expressed. |
| Function | Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2. Hydrolyzes adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Hydrolyzes 3-indolepropionic acyl-adenylate, tryptamine adenosine phosphoramidate monoester and other fluorogenic purine nucleoside tryptamine phosphoramidates in vitro. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex. Also exhibits SUMO-specific isopeptidase activity, deconjugating SUMO1 from RGS17. Deconjugates SUMO1 from RANGAP1. |
| Protein Name | Adenosine 5'-Monophosphoramidase Hint1Desumoylating Isopeptidase Hint1Histidine Triad Nucleotide-Binding Protein 1Protein Kinase C Inhibitor 1Protein Kinase C-Interacting Protein 1Pkci-1 |
| Database Links | Reactome: R-HSA-9824594Reactome: R-HSA-9825892Reactome: R-HSA-9856649 |
| Cellular Localisation | CytoplasmNucleusInteraction With Cdk7 Leads To A More Nuclear Localization |
| Alternative Antibody Names | Anti-Adenosine 5'-Monophosphoramidase Hint1 antibodyAnti-Desumoylating Isopeptidase Hint1 antibodyAnti-Histidine Triad Nucleotide-Binding Protein 1 antibodyAnti-Protein Kinase C Inhibitor 1 antibodyAnti-Protein Kinase C-Interacting Protein 1 antibodyAnti-Pkci-1 antibodyAnti-HINT1 antibodyAnti-HINT antibodyAnti-PKCI1 antibodyAnti-PRKCNH1 antibody |
Information sourced from Uniprot.org