![Immunofluorescence analysis of L929 cells using [KO Validated] HIF1AN/FIH1 Rabbit polyclonal antibody (STJ27419) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101116/388432/STJ27419_2__98546.1713157934.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] HIF1AN/FIH1 Rabbit polyclonal antibody (STJ27419) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry analysis of HIF1AN/FIH1 in paraffin-embedded Rat kidney tissue using [KO Validated] HIF1AN/FIH1 Rabbit polyclonal antibody (STJ27419) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101116/388433/STJ27419_3__73811.1713157934.jpg?c=1 "Immunohistochemistry analysis of HIF1AN/FIH1 in paraffin-embedded Rat kidney tissue using [KO Validated] HIF1AN/FIH1 Rabbit polyclonal antibody (STJ27419) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of HIF1AN/FIH1 in paraffin-embedded Human breast cancer tissue using [KO Validated] HIF1AN/FIH1 Rabbit polyclonal antibody (STJ27419) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101116/388434/STJ27419_4__81613.1713157935.jpg?c=1 "Immunohistochemistry analysis of HIF1AN/FIH1 in paraffin-embedded Human breast cancer tissue using [KO Validated] HIF1AN/FIH1 Rabbit polyclonal antibody (STJ27419) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of HIF1AN/FIH1 in paraffin-embedded Mouse kidney tissue using [KO Validated] HIF1AN/FIH1 Rabbit polyclonal antibody (STJ27419) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101116/388435/STJ27419_5__83902.1713157936.jpg?c=1 "Immunohistochemistry analysis of HIF1AN/FIH1 in paraffin-embedded Mouse kidney tissue using [KO Validated] HIF1AN/FIH1 Rabbit polyclonal antibody (STJ27419) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Western blot analysis of lysates from wild type (WT) and HIF1AN/FIH1 knockout (KO) 293T cells, using [KO Validated] HIF1AN/FIH1 Rabbit polyclonal antibody (STJ27419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101116/388436/STJ27419_6__63181.1713157937.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HIF1AN/FIH1 knockout (KO) 293T cells, using [KO Validated] HIF1AN/FIH1 Rabbit polyclonal antibody (STJ27419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of various lysates using [KO Validated] HIF1AN/FIH1 Rabbit polyclonal antibody (STJ27419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101116/388437/STJ27419_7__82255.1713157937.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] HIF1AN/FIH1 Rabbit polyclonal antibody (STJ27419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
Anti-HIF1AN antibody (1-349) (STJ27419)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal HIF1AN/FIH1 (1-349) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HIF1AN |
| Gene ID | 55662 |
| Uniprot ID | HIF1N_HUMAN |
| Immunogen Region | 1-349 |
| Immunogen Sequence | MAATAAEAVASGSGEPREEA GALGPAWDESQLRSYSFPTR PIPRLSQSDPRAEELIENEE PVVLTDTNLVYPALKWDLEY LQENIGNGDFSVYSASTHKF LYYDEKKMANFQNFKPRSNR EEMKFHEFVEKLQDIQQRGG EERLYLQQTLNDTVGRKIVM DFLGFNWNWINKQQGKRGWG QLTSNLLLIGMEGNVTPAHY DEQQNFFAQIKGYKRCILFP PDQFECLYPYPVHHPCDRQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human HIF1AN/FIH1 (NP_060372.2). |
Additional Info
| Function | Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. |
| Protein Name | Hypoxia-Inducible Factor 1-Alpha InhibitorFactor Inhibiting Hif-1Fih-1Hypoxia-Inducible Factor Asparagine Hydroxylase |
| Database Links | Reactome: R-HSA-1234174 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionMainly Cytoplasmic LocalizationBut Interaction With Notch1 Results In Nuclear Localization And Interaction With Abpa3 Results In Perinuclear Localization In Macrophages |
| Alternative Antibody Names | Anti-Hypoxia-Inducible Factor 1-Alpha Inhibitor antibodyAnti-Factor Inhibiting Hif-1 antibodyAnti-Fih-1 antibodyAnti-Hypoxia-Inducible Factor Asparagine Hydroxylase antibodyAnti-HIF1AN antibodyAnti-FIH1 antibody |
Information sourced from Uniprot.org